Design of a Fast, Efficient and Controlled DNA Shearing System Based on Lateral Acoustic Waves

Date
2012
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract

With the continuous research and advances in Deoxyribonucleic acid (DNA) sequencing technologies, the need for an efficient DNA shearing system has increased more than ever before. In this thesis, we propose a fast, efficient and controlled DNA shearing system based on a uniquely designed ultrasonic transducer, called Fresnel Annular Sector Actuator (FASA). Based on the simulation and experimental results, a circular array of four 90°-FASA elements is chosen as the basic unit for the proposed shearing system. DNA is successfully sheared from 300 to 1500 base-pair lengths. The shearing performance of the system is independent of the source of DNA over a large range of concentrations of the DNA. Finally, multiple FASA elements, excited by separate BF-signals, are used to increase the throughput of the proposed shearing system.

Description
Degree
Master of Science
Type
Thesis
Keywords
Applied sciences, Biomedical engineering, Electrical engineering
Citation

Dev, Kapil. "Design of a Fast, Efficient and Controlled DNA Shearing System Based on Lateral Acoustic Waves." (2012) Master’s Thesis, Rice University. https://hdl.handle.net/1911/70227.

Has part(s)
Forms part of
Published Version
Rights
Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
Link to license
Citable link to this page