Quantifying spectral changes experienced by plasmonic nanoparticles in a cellular environment to inform biomedical nanoparticle design

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dc.citation.firstpage454en_US
dc.citation.journalTitleNanoscale Research Lettersen_US
dc.citation.volumeNumber9en_US
dc.contributor.authorChen, Allen L.en_US
dc.contributor.authorHu, Ying S.en_US
dc.contributor.authorJackson, Meredith A.en_US
dc.contributor.authorLin, Adam Y.en_US
dc.contributor.authorYoung, Joseph K.en_US
dc.contributor.authorLangsner, Robert J.en_US
dc.contributor.authorDrezek, Rebekah A.en_US
dc.date.accessioned2014-10-30T19:33:32Zen_US
dc.date.available2014-10-30T19:33:32Zen_US
dc.date.issued2014en_US
dc.description.abstractMetal nanoparticles (NPs) scatter and absorb light in precise, designable ways, making them agile candidates for a variety of biomedical applications. When NPs are introduced to a physiological environment and interact with cells, their physicochemical properties can change as proteins adsorb on their surface and they agglomerate within intracellular endosomal vesicles. Since the plasmonic properties of metal NPs are dependent on their geometry and local environment, these physicochemical changes may alter the NPs' plasmonic properties, on which applications such as plasmonic photothermal therapy and photonic gene circuits are based. Here we systematically study and quantify how metal NPs' optical spectra change upon introduction to a cellular environment in which NPs agglomerate within endosomal vesicles. Using darkfield hyperspectral imaging, we measure changes in the peak wavelength, broadening, and distribution of 100-nm spherical gold NPs' optical spectra following introduction to human breast adenocarcinoma Sk-Br-3 cells as a function of NP exposure dose and time. On a cellular level, spectra shift up to 78.6 ± 23.5 nm after 24 h of NP exposure. Importantly, spectra broaden with time, achieving a spectral width of 105.9 ± 11.7 nm at 95% of the spectrum's maximum intensity after 24 h. On an individual intracellular NP cluster (NPC) level, spectra also show significant shifting, broadening, and heterogeneity after 24 h. Cellular transmission electron microscopy (TEM) and electromagnetic simulations of NPCs support the trends in spectral changes we measured. These quantitative data can help guide the design of metal NPs introduced to cellular environments in plasmonic NP-mediated biomedical technologies.en_US
dc.identifier.citationChen, Allen L., Hu, Ying S., Jackson, Meredith A., et al.. "Quantifying spectral changes experienced by plasmonic nanoparticles in a cellular environment to inform biomedical nanoparticle design." <i>Nanoscale Research Letters,</i> 9, (2014) Springer: 454. http://dx.doi.org/10.1186/1556-276X-9-454.en_US
dc.identifier.doihttp://dx.doi.org/10.1186/1556-276X-9-454en_US
dc.identifier.urihttps://hdl.handle.net/1911/77672en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsThis article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordnano-bio interactionsen_US
dc.subject.keywordplasmon resonanceen_US
dc.subject.keywordhyperspectral imagingen_US
dc.subject.keywordgold nanoparticlesen_US
dc.subject.keywordcellsen_US
dc.subject.keywordspectral analysisen_US
dc.subject.keywordnanomedicineen_US
dc.titleQuantifying spectral changes experienced by plasmonic nanoparticles in a cellular environment to inform biomedical nanoparticle designen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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