Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras

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dc.citation.firstpage645
dc.citation.issueNumber9
dc.citation.journalTitleACS Synthetic Biology
dc.citation.lastpage651
dc.citation.volumeNumber3
dc.contributor.authorShis, David L.
dc.contributor.authorHussain, Faiza
dc.contributor.authorMeinhardt, Sarah
dc.contributor.authorSwint-Kruse, Liskin
dc.contributor.authorBennett, Matthew R.
dc.date.accessioned2016-02-02T19:17:43Z
dc.date.available2016-02-02T19:17:43Z
dc.date.issued2014
dc.description.abstractIn prokaryotes, the construction of synthetic, multi-input promoters is constrained by the number of transcription factors that can simultaneously regulate a single promoter. This fundamental engineering constraint is an obstacle to synthetic biologists because it limits the computational capacity of engineered gene circuits. Here, we demonstrate that complex multi-input transcriptional logic gating can be achieved through the use of ligand-inducible chimeric transcription factors assembled from the LacI/GalR family. These modular chimeras each contain a ligand-binding domain and a DNA-binding domain, both of which are chosen from a library of possibilities. When two or more chimeras have the same DNA-binding domain, they independently and simultaneously regulate any promoter containing the appropriate operator site. In this manner, simple transcriptional AND gating is possible through the combination of two chimeras, and multiple-input AND gating is possible with the simultaneous use of three or even four chimeras. Furthermore, we demonstrate that orthogonal DNA-binding domains and their cognate operators allow the coexpression of multiple, orthogonal AND gates. Altogether, this work provides synthetic biologists with novel, ligand-inducible logic gates and greatly expands the possibilities for engineering complex synthetic gene circuits.
dc.identifier.citationShis, David L., Hussain, Faiza, Meinhardt, Sarah, et al.. "Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras." <i>ACS Synthetic Biology,</i> 3, no. 9 (2014) American Chemical Society: 645-651. http://dx.doi.org/10.1021/sb500262f.
dc.identifier.doihttp://dx.doi.org/10.1021/sb500262f
dc.identifier.urihttps://hdl.handle.net/1911/88299
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.rightsThis is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
dc.rights.urihttp://pubs.acs.org/page/policy/authorchoice_termsofuse.html
dc.titleModular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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