Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective

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dc.citation.firstpage96003en_US
dc.citation.issueNumber9en_US
dc.citation.journalTitleJournal of Biomedical Opticsen_US
dc.citation.volumeNumber18en_US
dc.contributor.authorKyrish, Matthewen_US
dc.contributor.authorDobbs, Jessicaen_US
dc.contributor.authorJain, Shalinien_US
dc.contributor.authorWang, Xiaoen_US
dc.contributor.authorYu, Dihuaen_US
dc.contributor.authorRichards-Kortum, Rebeccaen_US
dc.contributor.authorTkaczyk, Tomasz S.en_US
dc.date.accessioned2013-09-13T16:48:13Zen_US
dc.date.available2014-09-14T05:10:03Zen_US
dc.date.issued2013-09en_US
dc.description.abstractIn order to diagnose cancer, a sample must be removed, prepared, and examined under a microscope, which is expensive, invasive, and time consuming. Fiber optic fluorescence endomicroscopy, where an image guide is used to obtain high-resolution images of tissue in vivo, has shown promise as an alternative to conventional biopsies. However, the resolution of standard endomicroscopy is limited by the fiber bundle sampling frequency and out-of-focus light. A system is presented which incorporates a plastic, achromatic objective to increase the sampling and which provides optical sectioning via structured illumination to reject background light. An image is relayed from the sample by a fiber bundle with the custom 2.1-mm outer diameter objective lens integrated to the distal tip. The objective is corrected for the excitation and the emission wavelengths of proflavine (452 and 515 nm). It magnifies the object onto the fiber bundle to improve the systemﾒs lateral resolution by increasing the sampling. The plastic lenses were fabricated via single-point diamond turning and assembled using a zero alignment technique. Ex vivo images of normal and neoplastic murine mammary tissues stained with proflavine are captured. The system achieves higher contrast and resolves smaller features than standard fluorescence endomicroscopy.en_US
dc.embargo.terms1 yearen_US
dc.identifier.citationKyrish, Matthew, Dobbs, Jessica, Jain, Shalini, et al.. "Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective." <i>Journal of Biomedical Optics,</i> 18, no. 9 (2013) Society of Photo-Optical Instrumentation Engineers: 96003. http://dx.doi.org/10.1117/1.JBO.18.9.096003.en_US
dc.identifier.doihttp://dx.doi.org/10.1117/1.JBO.18.9.096003en_US
dc.identifier.urihttps://hdl.handle.net/1911/71908en_US
dc.language.isoengen_US
dc.publisherSociety of Photo-Optical Instrumentation Engineersen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.subject.keywordendomicroscopeen_US
dc.subject.keywordachromatic objectiveen_US
dc.subject.keywordcancer diagnosisen_US
dc.subject.keywordfluorescenceen_US
dc.subject.keywordprecision machiningen_US
dc.titleNeedle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objectiveen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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