Accurate Quantification of Disease Markers in Human Serum Using Iron Oxide Nanoparticle-linked Immunosorbent Assay

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dc.citation.firstpage1353en_US
dc.citation.issueNumber9en_US
dc.citation.journalTitleTheranosticsen_US
dc.citation.lastpage1361en_US
dc.citation.volumeNumber6en_US
dc.contributor.authorZhang, Linlinen_US
dc.contributor.authorTong, Shengen_US
dc.contributor.authorZhou, Junen_US
dc.contributor.authorBao, Gangen_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2016-07-06T19:28:53Zen_US
dc.date.available2016-07-06T19:28:53Zen_US
dc.date.issued2016en_US
dc.description.abstractAccurate and reliable quantification of biomarkers in the blood is essential in disease screening and diagnosis. Here we describe an iron oxide nanoparticle (IONP)-linked immunosorbent assay (ILISA) for detecting biomolecules in human serum. Sandwich ILISA was optimized for the detection of four important serological markers, IgA, IgG, IgM, and C-reactive protein (CRP), and assessed with normal sera, simulated disease-state sera and the serum samples from patients infected with West Nile virus (WNV) or human herpes virus (HHV). Our study shows that using the detection assay formulated with 18.8 nm wüstite nanocrystals, ILISA can achieve sub-picomolar detection sensitivity, and all four markers can be accurately quantified over a large dynamic range. In addition, ILISA is not susceptible to variations in operating procedures and shows better linearity and higher stability compared with ELISA, which facilitates its integration into detection methods suitable for point of care. Our results demonstrate that ILISA is a simple and versatile nanoplatform for highly sensitive and reliable detection of serological biomarkers in biomedical research and clinical applications.en_US
dc.identifier.citationZhang, Linlin, Tong, Sheng, Zhou, Jun, et al.. "Accurate Quantification of Disease Markers in Human Serum Using Iron Oxide Nanoparticle-linked Immunosorbent Assay." <i>Theranostics,</i> 6, no. 9 (2016) Ivyspring: 1353-1361. http://dx.doi.org/10.7150/thno.16093.en_US
dc.identifier.doihttp://dx.doi.org/10.7150/thno.16093en_US
dc.identifier.urihttps://hdl.handle.net/1911/90808en_US
dc.language.isoengen_US
dc.publisherIvyspringen_US
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/en_US
dc.subject.keywordiron oxide nanoparticleen_US
dc.subject.keywordimmunosorbent assayen_US
dc.subject.keywordserological biomarkeren_US
dc.subject.keywordquantificationen_US
dc.titleAccurate Quantification of Disease Markers in Human Serum Using Iron Oxide Nanoparticle-linked Immunosorbent Assayen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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