A novel tailed primer nucleic acid test for detection of HPV 16, 18 and 45 DNA at the point of care

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dc.citation.articleNumber20397en_US
dc.citation.journalTitleScientific Reportsen_US
dc.citation.volumeNumber13en_US
dc.contributor.authorChang, Megan M.en_US
dc.contributor.authorMa, Arielen_US
dc.contributor.authorNovak, Emilie Newshamen_US
dc.contributor.authorBarra, Mariaen_US
dc.contributor.authorKundrod, Kathryn A.en_US
dc.contributor.authorMontealegre, Jane Richardsen_US
dc.contributor.authorScheurer, Michael E.en_US
dc.contributor.authorCastle, Philip E.en_US
dc.contributor.authorSchmeler, Kathleenen_US
dc.contributor.authorRichards-Kortum, Rebeccaen_US
dc.date.accessioned2024-05-03T15:51:20Zen_US
dc.date.available2024-05-03T15:51:20Zen_US
dc.date.issued2023en_US
dc.description.abstractCervical cancer is a leading cause of death for women in low-resource settings despite being preventable through human papillomavirus (HPV) vaccination, early detection, and treatment of precancerous lesions. The World Health Organization recommends high-risk HPV (hrHPV) as the preferred cervical cancer screening strategy, which is difficult to implement in low-resource settings due to high costs, reliance on centralized laboratory infrastructure, and long sample-to-answer times. To help meet the need for rapid, low-cost, and decentralized cervical cancer screening, we developed tailed primer isothermal amplification and lateral flow detection assays for HPV16, HPV18, and HPV45 DNA. We translated these assays into a self-contained cartridge to achieve multiplexed detection of three hrHPV genotypes in a disposable cartridge. The developed test achieves clinically relevant limits of detection of 50–500 copies per reaction with extracted genomic DNA from HPV-positive cells. Finally, we performed sample-to-answer testing with direct lysates of HPV-negative and HPV-positive cell lines and demonstrated consistent detection of HPV16, HPV18, and HPV45 with 5000–50,000 cells/mL in < 35 min. With additional optimization to improve cartridge reliability, incorporation of additional hrHPV types, and validation with clinical samples, the assay could serve as a point-of-care HPV DNA test that improves access to cervical cancer screening in low-resource settings.en_US
dc.identifier.citationChang, M. M., Ma, A., Novak, E. N., Barra, M., Kundrod, K. A., Montealegre, J. R., Scheurer, M. E., Castle, P. E., Schmeler, K., & Richards-Kortum, R. (2023). A novel tailed primer nucleic acid test for detection of HPV 16, 18 and 45 DNA at the point of care. Scientific Reports, 13(1), 20397. https://doi.org/10.1038/s41598-023-47582-yen_US
dc.identifier.digitals41598-023-47582-yen_US
dc.identifier.doihttps://doi.org/10.1038/s41598-023-47582-yen_US
dc.identifier.urihttps://hdl.handle.net/1911/115626en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license. Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleA novel tailed primer nucleic acid test for detection of HPV 16, 18 and 45 DNA at the point of careen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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