Molecular machines open cell membranes

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dc.citation.firstpage567en_US
dc.citation.journalTitleNatureen_US
dc.citation.lastpage572en_US
dc.citation.volumeNumber548en_US
dc.contributor.authorGarcía-López, Víctoren_US
dc.contributor.authorChen, Fangen_US
dc.contributor.authorNilewski, Lizanne G.en_US
dc.contributor.authorDuret, Guillaumeen_US
dc.contributor.authorAliyan, Amiren_US
dc.contributor.authorKolomeisky, Anatoly B.en_US
dc.contributor.authorRobinson, Jacob T.en_US
dc.contributor.authorWang, Gufengen_US
dc.contributor.authorPal, Roberten_US
dc.contributor.authorTour, James M.en_US
dc.date.accessioned2017-11-15T14:12:33Zen_US
dc.date.available2017-11-15T14:12:33Zen_US
dc.date.issued2017en_US
dc.description.abstractBeyond the more common chemical delivery strategies, several physical techniques are used to open the lipid bilayers of cellular membranes[1]. These include using electric[2] and magnetic[3] fields, temperature[4], ultrasound[5] or light[6] to introduce compounds into cells, to release molecular species from cells or to selectively induce programmed cell death (apoptosis) or uncontrolled cell death (necrosis). More recently, molecular motors and switches that can change their conformation in a controlled manner in response to external stimuli have been used to produce mechanical actions on tissue for biomedical applications[7,8,9]. Here we show that molecular machines can drill through cellular bilayers using their molecular-scale actuation, specifically nanomechanical action. Upon physical adsorption of the molecular motors onto lipid bilayers and subsequent activation of the motors using ultraviolet light, holes are drilled in the cell membranes. We designed molecular motors and complementary experimental protocols that use nanomechanical action to induce the diffusion of chemical species out of synthetic vesicles, to enhance the diffusion of traceable molecular machines into and within live cells, to induce necrosis and to introduce chemical species into live cells. We also show that, by using molecular machines that bear short peptide addends, nanomechanical action can selectively target specific cell-surface recognition sites. Beyond the in vitro applications demonstrated here, we expect that molecular machines could also be used in vivo, especially as their design progresses to allow two-photon, near-infrared and radio-frequency activation[10]en_US
dc.identifier.citationGarcía-López, Víctor, Chen, Fang, Nilewski, Lizanne G., et al.. "Molecular machines open cell membranes." <i>Nature,</i> 548, (2017) Springer Nature: 567-572. https://doi.org/10.1038/nature23657.en_US
dc.identifier.digital295118_2_merged_1489432345en_US
dc.identifier.doihttps://doi.org/10.1038/nature23657en_US
dc.identifier.urihttps://hdl.handle.net/1911/98813en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer Nature.en_US
dc.titleMolecular machines open cell membranesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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