Evaluation of Endothelial Cells Differentiated from Amniotic Fluid-Derived Stem Cells

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dc.citation.firstpage1123en_US
dc.citation.issueNumber12-Noven_US
dc.citation.journalTitleTissue Engineering: Part Aen_US
dc.citation.lastpage1131en_US
dc.citation.volumeNumber18en_US
dc.contributor.authorBenavides, Omar M.en_US
dc.contributor.authorPetsche, Jennifer J.en_US
dc.contributor.authorMoise, Kenneth J. Jr.en_US
dc.contributor.authorJohnson, Anthonyen_US
dc.contributor.authorJacot, Jeffrey G.en_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2013-07-12T21:05:34Zen_US
dc.date.available2013-07-12T21:05:34Zen_US
dc.date.issued2012en_US
dc.description.abstractAmniotic fluid holds great promise as a stem cell source, especially in neonatal applications where autologous cells can be isolated and used. This study examined chemical-mediated differentiation of amniotic fluid-derived stem cells (AFSC) into endothelial cells and verified the function of AFSC-derived endothelial cells (AFSC-EC). AFSC were isolated from amniotic fluid obtained from second trimester amnioreduction as part of therapeutic intervention from pregnancies affected with twin-twin transfusion syndrome. Undifferentiated AFSC were of normal karyotype with a subpopulation of cells positive for the embryonic stem cell marker SSEA4, hematopoietic stem cell marker c-kit, and mesenchymal stem cell markers CD29, CD44, CD73, CD90, and CD105. Additionally, these cells were negative for the endothelial marker CD31 and hematopoietic differentiation marker CD45. AFSC were cultured in endothelial growth media with concentrations of vascular endothelial growth factor (VEGF) ranging from 1 to 100 ng/mL. After 2 weeks, AFSC-EC expressed von Willebrand factor, endothelial nitric oxide synthase, CD31, VE-cadherin, and VEGF receptor 2. Additionally, the percentage of cells expressing CD31 was positively correlated with VEGF concentration up to 50 ng/mL, with no increase at higher concentrations. AFSC-EC showed a decrease in stem cells markers c-kit and SSEA4 and were morphologically similar to human umbilical vein endothelial cells (HUVEC). In functional assays, AFSC-EC formed networks and metabolized acetylated low-density lipoprotein, also characteristic of HUVEC. Nitrate levels for AFSC-EC, an indirect measure of nitric oxide synthesis, were significantly higher than undifferentiated controls and significantly lower than HUVEC. These results indicate that AFSC can differentiate into functional endothelial-like cells and may have the potential to provide vascularization for constructs used in regenerative medicine strategies.en_US
dc.embargo.termsnoneen_US
dc.identifier.citationBenavides, Omar M., Petsche, Jennifer J., Moise, Kenneth J. Jr., et al.. "Evaluation of Endothelial Cells Differentiated from Amniotic Fluid-Derived Stem Cells." <i>Tissue Engineering: Part A,</i> 18, no. 11-12 (2012) Mary Ann Liebert, Inc.: 1123-1131. http://dx.doi.org/10.1089/ten.tea.2011.0392.en_US
dc.identifier.doihttp://dx.doi.org/10.1089/ten.tea.2011.0392en_US
dc.identifier.urihttps://hdl.handle.net/1911/71554en_US
dc.language.isoengen_US
dc.publisherMary Ann Liebert, Inc.en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.titleEvaluation of Endothelial Cells Differentiated from Amniotic Fluid-Derived Stem Cellsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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