Production of succinic acid by engineered E. coli strains using soybean carbohydrates as feedstock under aerobic fermentation conditions

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dc.citation.firstpage398en_US
dc.citation.journalTitleBioresource Technologyen_US
dc.citation.lastpage405en_US
dc.citation.volumeNumber130en_US
dc.contributor.authorThakker, Chandreshen_US
dc.contributor.authorSan, Ka-Yiuen_US
dc.contributor.authorBennett, George N.en_US
dc.date.accessioned2014-02-14T21:19:56Zen_US
dc.date.available2014-02-14T21:19:56Zen_US
dc.date.issued2013en_US
dc.description.abstractEscherichia coli strains HL2765 and HL27659k harboring pRU600 and pKK313 were examined for succinate production under aerobic conditions using galactose, sucrose, raffinose, stachyose, and mixtures of these sugars extracted from soybean meal and soy solubles. HL2765(pKK313)(pRU600) and HL27659k(pKK313)(pRU600) consumed 87 mMand 98 mMhexose of soybean meal extract and produced 83 mM and 95 mM succinate, respectively. While using soy solubles extract, HL2765(pKK313)(pRU600) and HL27659k(pKK313)(pRU600) consumed 160 mM and 187 mM hexose and produced 158 mM and 183 mMsuccinate, respectively. Succinate yield of HL2765(pKK313)(pRU600)was low as compared to that of HL27659k(pKK313)(pRU600) while using acid hydrolysate of soybean meal or soy solubles extracts. Maximum succinate production of 312 mMwith a molar yield of 0.82 mol/mol hexose was obtained using soy solubles hydrolysate by HL27659k(pKK313)(pRU600). This study demonstrated the use of soluble carbohydrates of the renewable feedstock, soybean as an inexpensive carbon source to produce succinate by fermentation.en_US
dc.identifier.citationThakker, Chandresh, San, Ka-Yiu and Bennett, George N.. "Production of succinic acid by engineered E. coli strains using soybean carbohydrates as feedstock under aerobic fermentation conditions." <i>Bioresource Technology,</i> 130, (2013) Elsevier: 398-405. http://dx.doi.org/10.1016/j.biortech.2012.10.154.en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.biortech.2012.10.154en_US
dc.identifier.urihttps://hdl.handle.net/1911/75477en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordEscherichia colien_US
dc.subject.keywordaerobic fermentationen_US
dc.subject.keywordsoybean carbohydratesen_US
dc.subject.keywordsoy solublesen_US
dc.titleProduction of succinic acid by engineered E. coli strains using soybean carbohydrates as feedstock under aerobic fermentation conditionsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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