Subcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70

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dc.citation.articleNumber30en_US
dc.citation.journalTitleVirology Journalen_US
dc.citation.volumeNumber14en_US
dc.contributor.authorZhang, Wentingen_US
dc.contributor.authorCai, Chengzhien_US
dc.contributor.authorLin, Lien_US
dc.contributor.authorTao, Yizhi Janeen_US
dc.contributor.authorJin, Meilinen_US
dc.contributor.orgBiosciencesen_US
dc.date.accessioned2018-12-20T18:56:38Zen_US
dc.date.available2018-12-20T18:56:38Zen_US
dc.date.issued2017en_US
dc.description.abstractBACKGROUND: Infectious salmon anemia virus (ISAV) is an important fish pathogen that causes high mortality in farmed Atlantic salmon. The ISAV genome consists of eight single-stranded, negative-sense RNA segments. The six largest segments contain one open reading frame (ORF) each, and encode three polymerase proteins, nucleoprotein, fusion protein, and hemagglutinin esterase protein. The two smallest segments contain more than one ORF each. The segment 7 encodes non-structural protein 1 (NS1) and nuclear export protein (NEP), while segment 8 encodes matrix protein 1 and 2 (M1 and M2). NS1 and M2 have been well known as antagonist of type I interferon. However, little is known about the characterization of M1 or NEP. In addition, heat shock cognate 70 (Hsc70) has been reported to interact with M1 and NEP of influenza viruses for the export of viral ribonucleoprotein (vRNP) via vRNP-M1-NEP complex, the goal of this study therefore was to characterize the subcellular localization and interactions of ISAV M1 and NEP as well as cellular Hsc70. RESULTS: When M1, NEP, and Hsc70 were individually expressed in the stripped snakehead (SSN-1) cells, we found that M1 protein was localized in both cytosol and nucleus of the cells, NEP was localized only in the cytosol and accumulated adjacent to the nucleus, while Hsc70 was localized throughout the cytosol, but not in the nucleus. However, when two of them were co-expressed, we found that both M1 and Hsc70 were co-localized with NEP in the cytosol and accumulated adjacent to the nucleus, while M1 and Hsc70 were still localized as they were expressed individually. Furthermore, pull-down assay was performed and showed that NEP could interact with both M1 and Hsc70, and M1-Hsc70 interaction was also observed although the interaction was weaker than that of NEP-Hsc70. CONCLUSION: Our study characterized the subcellular localization and interactions of three proteins including M1 and NEP of ISAV, and Hsc70. These data will help towards a better understanding of the life cycle of ISAV, especially the process of vRNP export.en_US
dc.identifier.citationZhang, Wenting, Cai, Chengzhi, Lin, Li, et al.. "Subcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70." <i>Virology Journal,</i> 14, (2017) BioMed Central: https://doi.org/10.1186/s12985-017-0702-z.en_US
dc.identifier.digitals12985-017-0702-zen_US
dc.identifier.doihttps://doi.org/10.1186/s12985-017-0702-zen_US
dc.identifier.urihttps://hdl.handle.net/1911/104926en_US
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordHeat shock cognate 70en_US
dc.subject.keywordInfectious salmon anemia virusen_US
dc.subject.keywordMatrix proteinen_US
dc.subject.keywordNuclear export proteinen_US
dc.subject.keywordProtein interactionen_US
dc.subject.keywordSubcellular localizationen_US
dc.titleSubcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70en_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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