Ultrafast laser surgery probe for sub-surface ablation to enable biomaterial injection in vocal folds
| dc.citation.articleNumber | 20554 | |
| dc.citation.journalTitle | Scientific Reports | |
| dc.citation.volumeNumber | 12 | |
| dc.contributor.author | Andrus, Liam | |
| dc.contributor.author | Jeon, Hamin | |
| dc.contributor.author | Pawlowski, Michal | |
| dc.contributor.author | Debord, Benoit | |
| dc.contributor.author | Gerome, Frederic | |
| dc.contributor.author | Benabid, Fetah | |
| dc.contributor.author | Mau, Ted | |
| dc.contributor.author | Tkaczyk, Tomasz | |
| dc.contributor.author | Ben-Yakar, Adela | |
| dc.date.accessioned | 2023-01-27T14:47:39Z | |
| dc.date.available | 2023-01-27T14:47:39Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Creation of sub-epithelial voids within scarred vocal folds via ultrafast laser ablation may help in localization of injectable therapeutic biomaterials towards an improved treatment for vocal fold scarring. Several ultrafast laser surgery probes have been developed for precise ablation of surface tissues; however, these probes lack the tight beam focusing required for sub-surface ablation in highly scattering tissues such as vocal folds. Here, we present a miniaturized ultrafast laser surgery probe designed to perform sub-epithelial ablation in vocal folds. The requirement of high numerical aperture for sub-surface ablation, in addition to the small form factor and side-firing architecture required for clinical use, made for a challenging optical design. An Inhibited Coupling guiding Kagome hollow core photonic crystal fiber delivered micro-Joule level ultrashort pulses from a high repetition rate fiber laser towards a custom-built miniaturized objective, producing a 1/e2 focal beam radius of 1.12 ± 0.10 μm and covering a 46 × 46 μm2 scan area. The probe could deliver up to 3.8 μJ pulses to the tissue surface at 40% transmission efficiency through the entire system, providing significantly higher fluences at the focal plane than were required for sub-epithelial ablation. To assess surgical performance, we performed ablation studies on freshly excised porcine hemi-larynges and found that large area sub-epithelial voids could be created within vocal folds by mechanically translating the probe tip across the tissue surface using external stages. Finally, injection of a model biomaterial into a 1 × 2 mm2 void created 114 ± 30 μm beneath the vocal fold epithelium surface indicated improved localization when compared to direct injection into the tissue without a void, suggesting that our probe may be useful for pre-clinical evaluation of injectable therapeutic biomaterials for vocal fold scarring therapy. With future developments, the surgical system presented here may enable treatment of vocal fold scarring in a clinical setting. | |
| dc.identifier.citation | Andrus, Liam, Jeon, Hamin, Pawlowski, Michal, et al.. "Ultrafast laser surgery probe for sub-surface ablation to enable biomaterial injection in vocal folds." <i>Scientific Reports,</i> 12, (2022) Springer Nature: https://doi.org/10.1038/s41598-022-24446-5. | |
| dc.identifier.digital | s41598-022-24446-5 | |
| dc.identifier.doi | https://doi.org/10.1038/s41598-022-24446-5 | |
| dc.identifier.uri | https://hdl.handle.net/1911/114297 | |
| dc.language.iso | eng | |
| dc.publisher | Springer Nature | |
| dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.title | Ultrafast laser surgery probe for sub-surface ablation to enable biomaterial injection in vocal folds | |
| dc.type | Journal article | |
| dc.type.dcmi | Text | |
| dc.type.publication | publisher version |
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