Adsorption and Unfolding of a Single Protein Triggers Nanoparticle Aggregation

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dc.citation.firstpage2103
dc.citation.issueNumber2
dc.citation.journalTitleACS Nano
dc.citation.lastpage2112
dc.citation.volumeNumber10
dc.contributor.authorDominguez-Medina, Sergio
dc.contributor.authorKisley, Lydia
dc.contributor.authorTauzin, Lawrence J.
dc.contributor.authorHoggard, Anneli
dc.contributor.authorShuang, Bo
dc.contributor.authorIndrasekara, A. Swarnapali D.S.
dc.contributor.authorChen, Sishan
dc.contributor.authorWang, Lin-Yung
dc.contributor.authorDerry, Paul J.
dc.contributor.authorLiopo, Anton
dc.contributor.authorZubarev, Eugene R.
dc.contributor.authorLandes, Christy F.
dc.contributor.authorLink, Stephan
dc.date.accessioned2016-04-05T20:14:03Z
dc.date.available2016-04-05T20:14:03Z
dc.date.issued2016
dc.description.abstractThe response of living systems to nanoparticles is thought to depend on the protein corona, which forms shortly after exposure to physiological fluids and which is linked to a wide array of pathophysiologies. A mechanistic understanding of the dynamic interaction between proteins and nanoparticles and thus the biological fate of nanoparticles and associated proteins is, however, often missing mainly due to the inadequacies in current ensemble experimental approaches. Through the application of a variety of single molecule and single particle spectroscopic techniques in combination with ensemble level characterization tools, we identified different interaction pathways between gold nanorods and bovine serum albumin depending on the protein concentration. Overall, we found that local changes in protein concentration influence everything from cancer cell uptake to nanoparticle stability and even protein secondary structure. We envision that our findings and methods will lead to strategies to control the associated pathophysiology of nanoparticle exposure in vivo.
dc.identifier.citationDominguez-Medina, Sergio, Kisley, Lydia, Tauzin, Lawrence J., et al.. "Adsorption and Unfolding of a Single Protein Triggers Nanoparticle Aggregation." <i>ACS Nano,</i> 10, no. 2 (2016) American Chemical Society: 2103-2112. http://dx.doi.org/10.1021/acsnano.5b06439.
dc.identifier.doihttp://dx.doi.org/10.1021/acsnano.5b06439
dc.identifier.urihttps://hdl.handle.net/1911/88853
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.rightsThis is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
dc.rights.urihttp://pubs.acs.org/page/policy/authorchoice_termsofuse.htmlen_US
dc.subject.keywordprotein corona
dc.subject.keywordnanorods
dc.subject.keywordsuperlocalization microscopy
dc.subject.keywordcorrelation spectroscopy
dc.subject.keywordsurface plasmon
dc.titleAdsorption and Unfolding of a Single Protein Triggers Nanoparticle Aggregation
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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