An inexpensive, customizable microscopy system for the automated quantification and characterization of multiple adherent cell types

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dc.citation.articleNumbere4937en_US
dc.citation.journalTitlePeerJen_US
dc.citation.volumeNumber6en_US
dc.contributor.authorAsthana, Vishwaratnen_US
dc.contributor.authorTang, Yuqien_US
dc.contributor.authorFerguson, Adamen_US
dc.contributor.authorBugga, Pallavien_US
dc.contributor.authorAsthana, Anantratnen_US
dc.contributor.authorEvans, Emily R.en_US
dc.contributor.authorChen, Allen L.en_US
dc.contributor.authorStern, Brett S.en_US
dc.contributor.authorDrezek, Rebekah A.en_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2018-09-26T14:52:42Zen_US
dc.date.available2018-09-26T14:52:42Zen_US
dc.date.issued2018en_US
dc.description.abstractCell quantification assays are essential components of most biological and clinical labs. However, many currently available quantification assays, including flow cytometry and commercial cell counting systems, suffer from unique drawbacks that limit their overall efficacy. In order to address the shortcomings of traditional quantification assays, we have designed a robust, low-cost, automated microscopy-based cytometer that quantifies individual cells in a multiwell plate using tools readily available in most labs. Plating and subsequent quantification of various dilution series using the automated microscopy-based cytometer demonstrates the single-cell sensitivity, near-perfect R2 accuracy, and greater than 5-log dynamic range of our system. Further, the microscopy-based cytometer is capable of obtaining absolute counts of multiple cell types in one well as part of a co-culture setup. To demonstrate this ability, we recreated an experiment that assesses the tumoricidal properties of primed macrophages on co-cultured tumor cells as a proof-of-principle test. The results of the experiment reveal that primed macrophages display enhanced cytotoxicity toward tumor cells while simultaneously losing the ability to proliferate, an example of a dynamic interplay between two cell populations that our microscopy-based cytometer is successfully able to elucidate.en_US
dc.identifier.citationAsthana, Vishwaratn, Tang, Yuqi, Ferguson, Adam, et al.. "An inexpensive, customizable microscopy system for the automated quantification and characterization of multiple adherent cell types." <i>PeerJ,</i> 6, (2018) PeerJ: https://doi.org/10.7717/peerj.4937.en_US
dc.identifier.digitalpeerj-4937en_US
dc.identifier.doihttps://doi.org/10.7717/peerj.4937en_US
dc.identifier.urihttps://hdl.handle.net/1911/102712en_US
dc.language.isoengen_US
dc.publisherPeerJen_US
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordcell quantificationen_US
dc.subject.keywordco-cultureen_US
dc.subject.keywordfluorescent microscopyen_US
dc.subject.keywordoptical cytometryen_US
dc.titleAn inexpensive, customizable microscopy system for the automated quantification and characterization of multiple adherent cell typesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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