Vital-dye-enhanced multimodal imaging of neoplastic progression in a mouse model of oral carcinogenesis

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dc.citation.firstpage126017en_US
dc.citation.issueNumber12en_US
dc.citation.journalTitleJournal of Biomedical Opticsen_US
dc.citation.volumeNumber18en_US
dc.contributor.authorHellebust, Anneen_US
dc.contributor.authorRosbach, Kelseyen_US
dc.contributor.authorWu, Jessica Kerenen_US
dc.contributor.authorNguyen, Jenniferen_US
dc.contributor.authorGillenwater, Annen_US
dc.contributor.authorVigneswaran, Nadarajahen_US
dc.contributor.authorRichards-Kortum, Rebeccaen_US
dc.date.accessioned2015-03-19T15:07:12Zen_US
dc.date.available2015-03-19T15:07:12Zen_US
dc.date.issued2013en_US
dc.description.abstractIn this longitudinal study, a mouse model of 4-nitroquinoline 1-oxide chemically induced tongue carcinogenesis was used to assess the ability of optical imaging with exogenous and endogenous contrast to detect neoplastic lesions in a heterogeneous mucosal surface. Widefield autofluorescence and fluorescence images of intact 2-NBDG-stained and proflavine-stained tissues were acquired at multiple time points in the carcinogenesis process. Confocal fluorescence images of transverse fresh tissue slices from the same specimens were acquired to investigate how changes in tissue microarchitecture affect widefield fluorescence images of intact tissue. Widefield images were analyzed to develop and evaluate an algorithm to delineate areas of dysplasia and cancer. A classification algorithm for the presence of neoplasia based on the mean fluorescence intensity of 2-NBDG staining and the standard deviation of the fluorescence intensity of proflavine staining was found to separate moderate dysplasia, severe dysplasia, and cancer from non-neoplastic regions of interest with 91% sensitivity and specificity. Results suggest this combination of noninvasive optical imaging modalities can be used in vivo to discriminate non-neoplastic from neoplastic tissue in this model with the potential to translate this technology to the clinic.en_US
dc.identifier.citationHellebust, Anne, Rosbach, Kelsey, Wu, Jessica Keren, et al.. "Vital-dye-enhanced multimodal imaging of neoplastic progression in a mouse model of oral carcinogenesis." <i>Journal of Biomedical Optics,</i> 18, no. 12 (2013) SPIE: 126017. http://dx.doi.org/10.1117/1.JBO.18.12.126017.en_US
dc.identifier.doihttp://dx.doi.org/10.1117/1.JBO.18.12.126017en_US
dc.identifier.urihttps://hdl.handle.net/1911/79389en_US
dc.language.isoengen_US
dc.publisherSPIEen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.subject.keywordoptical imagingen_US
dc.subject.keywordfluorescenceen_US
dc.subject.keywordmicroscopyen_US
dc.subject.keywordcarcinogenesisen_US
dc.subject.keywordmouse cancer modelen_US
dc.subject.keywordcontrast agentsen_US
dc.titleVital-dye-enhanced multimodal imaging of neoplastic progression in a mouse model of oral carcinogenesisen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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