Measuring the Grafting Density of Nanoparticles in Solution by Analytical Ultracentrifugation and Total Organic Carbon Analysis

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dc.citation.firstpage9238
dc.citation.issueNumber21
dc.citation.journalTitleAnalytical Chemistry
dc.citation.lastpage9245
dc.citation.volumeNumber84
dc.contributor.authorBenoit, Denise N.
dc.contributor.authorZhu, Huiguang
dc.contributor.authorLilierose, Michael H.
dc.contributor.authorVerm, Raymond A.
dc.contributor.authorAli, Naushaba
dc.contributor.authorMorrison, Adam N.
dc.contributor.authorFortner, John D.
dc.contributor.authorAvendano, Carolina
dc.contributor.authorColvin, Vicki L.
dc.date.accessioned2017-08-02T14:52:15Z
dc.date.available2017-08-02T14:52:15Z
dc.date.issued2012
dc.description.abstractMany of the solution phase properties of nanoparticles, such as their colloidal stability and hydrodynamic diameter, are governed by the number of stabilizing groups bound to the particle surface (i.e., grafting density). Here, we show how two techniques, analytical ultracentrifugation (AUC) and total organic carbon analysis (TOC), can be applied separately to the measurement of this parameter. AUC directly measures the density of nanoparticle–polymer conjugates while TOC provides the total carbon content of its aqueous dispersions. When these techniques are applied to model gold nanoparticles capped with thiolated poly(ethylene glycol), the measured grafting densities across a range of polymer chain lengths, polymer concentrations, and nanoparticle diameters agree to within 20%. Moreover, the measured grafting densities correlate well with the polymer content determined by thermogravimetric analysis of solid conjugate samples. Using these tools, we examine the particle core diameter, polymer chain length, and polymer solution concentration dependence of nanoparticle grafting densities in a gold nanoparticle–poly(ethylene glycol) conjugate system.
dc.identifier.citationBenoit, Denise N., Zhu, Huiguang, Lilierose, Michael H., et al.. "Measuring the Grafting Density of Nanoparticles in Solution by Analytical Ultracentrifugation and Total Organic Carbon Analysis." <i>Analytical Chemistry,</i> 84, no. 21 (2012) American Chemical Society: 9238-9245. https://doi.org/10.1021/ac301980a.
dc.identifier.digitalMeasuring_Grafting_Density
dc.identifier.doihttps://doi.org/10.1021/ac301980a
dc.identifier.urihttps://hdl.handle.net/1911/96164
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.
dc.titleMeasuring the Grafting Density of Nanoparticles in Solution by Analytical Ultracentrifugation and Total Organic Carbon Analysis
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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