Role of Static and Dynamic Obstacles in the Protein Search for Targets on DNA

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dc.citation.journalTitleThe Journal of Physical Chemistry B
dc.contributor.authorShvets, Alexey
dc.contributor.authorKochugaeva, Maria
dc.contributor.authorKolomeisky, Anatoly B.
dc.contributor.orgCenter for Theoretical Biological Physics
dc.date.accessioned2016-01-14T20:01:03Z
dc.date.available2016-01-14T20:01:03Z
dc.date.issued2015
dc.description.abstractProtein search for specific sequences on DNA marks the beginning of major biological processes. Experiments indicate that proteins find and recognize their targets quickly and efficiently. Because of the large number of experimental and theoretical investigations, there is a reasonable understanding of the protein search processes in purifiedﾠin vitroﾠsystems. However, the situation is much more complex in live cells where multiple biochemical and biophysical processes can interfere with the protein search dynamics. In this study, we develop a theoretical method that explores the effect of crowding on DNA chains during the protein search. More specifically, the role of static and dynamic obstacles is investigated. The method employs a discrete-state stochastic framework that accounts for most relevant physical and chemical processes in the system. Our approach also provides an analytical description for all dynamic properties. It is found that the presence of the obstacles can significantly modify the protein search dynamics. This effect depends on the size of the obstacles, on the spatial positions of the target and the obstacles, on the nature of the search regime, and on the dynamic nature of the obstacles. It is argued that the crowding on DNA can accelerate or slow down the protein search dynamics depending on these factors. A comparison with existing experimental and theoretical results is presented. Theoretical results are discussed using simple physical-chemical arguments, and they are also tested with extensive Monte Carlo computer simulations.
dc.identifier.citationShvets, Alexey, Kochugaeva, Maria and Kolomeisky, Anatoly B.. "Role of Static and Dynamic Obstacles in the Protein Search for Targets on DNA." <i>The Journal of Physical Chemistry B,</i> (2015) America Chemical Society: http://dx.doi.org/10.1021/acs.jpcb.5b09814.
dc.identifier.doihttp://dx.doi.org/10.1021/acs.jpcb.5b09814
dc.identifier.urihttps://hdl.handle.net/1911/87816
dc.language.isoeng
dc.publisherAmerica Chemical Society
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.
dc.subject.keywordprotein-DNA interactions
dc.subject.keywordcrowding
dc.subject.keyworddiscrete-state stochastic models
dc.subject.keywordfirst-passage processes
dc.titleRole of Static and Dynamic Obstacles in the Protein Search for Targets on DNA
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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