Multiplexing cell-cell communication

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dc.citation.articleNumbere9618en_US
dc.citation.issueNumber7en_US
dc.citation.journalTitleMolecular Systems Biologyen_US
dc.citation.volumeNumber16en_US
dc.contributor.authorSexton, John T.en_US
dc.contributor.authorTabor, Jeffrey J.en_US
dc.contributor.orgBioengineeringen_US
dc.contributor.orgBiosciencesen_US
dc.date.accessioned2020-08-14T20:13:37Zen_US
dc.date.available2020-08-14T20:13:37Zen_US
dc.date.issued2020en_US
dc.description.abstractThe engineering of advanced multicellular behaviors, such as the programmed growth of biofilms or tissues, requires cells to communicate multiple aspects of physiological information. Unfortunately, few cell-cell communication systems have been developed for synthetic biology. Here, we engineer a genetically encoded channel selector device that enables a single communication system to transmit two separate intercellular conversations. Our design comprises multiplexer and demultiplexer sub-circuits constructed from a total of 12 CRISPRi-based transcriptional logic gates, an acyl homoserine lactone-based communication module, and three inducible promoters that enable small molecule control over the conversations. Experimentally parameterized mathematical models of the sub-components predict the steady state and dynamical performance of the full system. Multiplexed cell-cell communication has applications in synthetic development, metabolic engineering, and other areas requiring the coordination of multiple pathways among a community of cells.en_US
dc.identifier.citationSexton, John T. and Tabor, Jeffrey J.. "Multiplexing cell-cell communication." <i>Molecular Systems Biology,</i> 16, no. 7 (2020) EMBO Press: https://doi.org/10.15252/msb.20209618.en_US
dc.identifier.doihttps://doi.org/10.15252/msb.20209618en_US
dc.identifier.urihttps://hdl.handle.net/1911/109221en_US
dc.language.isoengen_US
dc.publisherEMBO Pressen_US
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordcell-cell communicationen_US
dc.subject.keywordCRISPRien_US
dc.subject.keywordgenetic circuit designen_US
dc.subject.keywordmultiplexersen_US
dc.subject.keywordsynthetic biologyen_US
dc.titleMultiplexing cell-cell communicationen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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