Evaluating the physicochemical effects of conjugating peptides into thermogelling hydrogels for regenerative biomaterials applications

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dc.citation.articleNumberrbab073
dc.citation.issueNumber6
dc.citation.journalTitleRegenerative Biomaterials
dc.citation.volumeNumber8
dc.contributor.authorPearce, Hannah A.
dc.contributor.authorJiang, Emily Y.
dc.contributor.authorSwain, Joseph W.R.
dc.contributor.authorNavara, Adam M.
dc.contributor.authorGuo, Jason L.
dc.contributor.authorKim, Yu Seon
dc.contributor.authorWoehr, Andrew
dc.contributor.authorHartgerink, Jeffrey D.
dc.contributor.authorMikos, Antonios G.
dc.date.accessioned2022-08-04T14:53:25Z
dc.date.available2022-08-04T14:53:25Z
dc.date.issued2021
dc.description.abstractThermogelling hydrogels, such as poly(N-isopropylacrylamide) [P(NiPAAm)], provide tunable constructs leveraged in many regenerative biomaterial applications. Recently, our lab developed the crosslinker poly(glycolic acid)-poly(ethylene glycol)-poly(glycolic acid)-di(but-2-yne-1,4-dithiol), which crosslinks P(NiPAAm-co-glycidyl methacrylate) via thiol-epoxy reaction and can be functionalized with azide-terminated peptides via alkyne-azide click chemistry. This study’s aim was to evaluate the impact of peptides on the physicochemical properties of the hydrogels. The physicochemical properties of the hydrogels including the lower critical solution temperature, crosslinking times, swelling, degradation, peptide release and cytocompatibility were evaluated. The gels bearing peptides increased equilibrium swelling indicating hydrophilicity of the hydrogel components. Comparable sol fractions were found for all groups, indicating that inclusion of peptides does not impact crosslinking. Moreover, the inclusion of a matrix metalloproteinase-sensitive peptide allowed elucidation of whether release of peptides from the network was driven by hydrolysis or enzymatic cleavage. The hydrophilicity of the network determined by the swelling behavior was demonstrated to be the most important factor in dictating hydrogel behavior over time. This study demonstrates the importance of characterizing the impact of additives on the physicochemical properties of hydrogels. These characteristics are key in determining design considerations for future in vitro and in vivo studies for tissue regeneration.
dc.identifier.citationPearce, Hannah A., Jiang, Emily Y., Swain, Joseph W.R., et al.. "Evaluating the physicochemical effects of conjugating peptides into thermogelling hydrogels for regenerative biomaterials applications." <i>Regenerative Biomaterials,</i> 8, no. 6 (2021) Oxford University Press: https://doi.org/10.1093/rb/rbab073.
dc.identifier.digitalrbab073
dc.identifier.doihttps://doi.org/10.1093/rb/rbab073
dc.identifier.urihttps://hdl.handle.net/1911/112977
dc.language.isoeng
dc.publisherOxford University Press
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleEvaluating the physicochemical effects of conjugating peptides into thermogelling hydrogels for regenerative biomaterials applications
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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