Equipment-Free Incubation of Recombinase Polymerase Amplification Reactions Using Body Heat

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dc.citation.issueNumber11en_US
dc.citation.journalTitlePLoS Oneen_US
dc.citation.lastpagee112146en_US
dc.citation.volumeNumber9en_US
dc.contributor.authorCrannell, Zachary Austinen_US
dc.contributor.authorRohrman, Brittanyen_US
dc.contributor.authorRichards-Kortum, Rebeccaen_US
dc.date.accessioned2014-12-05T18:35:05Zen_US
dc.date.available2014-12-05T18:35:05Zen_US
dc.date.issued2014en_US
dc.description.abstractThe development of isothermal amplification platforms for nucleic acid detection has the potential to increase access to molecular diagnostics in low resource settings; however, simple, low-cost methods for heating samples are required to perform reactions. In this study, we demonstrated that human body heat may be harnessed to incubate recombinase polymerase amplification (RPA) reactions for isothermal amplification of HIV-1 DNA. After measuring the temperature of mock reactions at 4 body locations, the axilla was chosen as the ideal site for comfortable, convenient incubation. Using commonly available materials, 3 methods for securing RPA reactions to the body were characterized. Finally, RPA reactions were incubated using body heat while control RPA reactions were incubated in a heat block. At room temperature, all reactions with 10 copies of HIV-1 DNA and 90% of reactions with 100 copies of HIV-1 DNA tested positive when incubated with body heat. In a cold room with an ambient temperature of 10 degrees Celsius, all reactions containing 10 copies or 100 copies of HIV-1 DNA tested positive when incubated with body heat. These results suggest that human body heat may provide an extremely low-cost solution for incubating RPA reactions in low resource settings.en_US
dc.identifier.citationCrannell, Zachary Austin, Rohrman, Brittany and Richards-Kortum, Rebecca. "Equipment-Free Incubation of Recombinase Polymerase Amplification Reactions Using Body Heat." <i>PLoS One,</i> 9, no. 11 (2014) Public Library of Science:-e112146 http://dx.doi.org/10.1371/journal.pone.0112146.en_US
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0112146en_US
dc.identifier.urihttps://hdl.handle.net/1911/78584en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleEquipment-Free Incubation of Recombinase Polymerase Amplification Reactions Using Body Heaten_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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