Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors

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dc.citation.articleNumber64en_US
dc.citation.journalTitleNature Communicationsen_US
dc.citation.volumeNumber9en_US
dc.contributor.authorChen, Yeen_US
dc.contributor.authorHo, Joanne M.L.en_US
dc.contributor.authorShis, David L.en_US
dc.contributor.authorGupta, Chinmayaen_US
dc.contributor.authorLong, Jamesen_US
dc.contributor.authorWagner, Daniel S.en_US
dc.contributor.authorOtt, Williamen_US
dc.contributor.authorJosić, Krešimiren_US
dc.contributor.authorBennett, Matthew R.en_US
dc.contributor.orgBioengineeringen_US
dc.contributor.orgBiosciencesen_US
dc.date.accessioned2018-07-02T18:19:43Zen_US
dc.date.available2018-07-02T18:19:43Zen_US
dc.date.issued2018en_US
dc.description.abstractOne challenge for synthetic biologists is the predictable tuning of genetic circuit regulatory components to elicit desired outputs. Gene expression driven by ligand-inducible transcription factor systems must exhibit the correct ON and OFF characteristics: appropriate activation and leakiness in the presence and absence of inducer, respectively. However, the dynamic range of a promoter (i.e., absolute difference between ON and OFF states) is difficult to control. We report a method that tunes the dynamic range of ligand-inducible promoters to achieve desired ON and OFF characteristics. We build combinatorial sets of AraC-and LasR-regulated promoters containing -10 and -35 sites from synthetic and Escherichia coli promoters. Four sequence combinations with diverse dynamic ranges were chosen to build multi-input transcriptional logic gates regulated by two and three ligand-inducible transcription factors (LacI, TetR, AraC, XylS, RhlR, LasR, and LuxR). This work enables predictable control over the dynamic range of regulatory components.en_US
dc.identifier.citationChen, Ye, Ho, Joanne M.L., Shis, David L., et al.. "Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors." <i>Nature Communications,</i> 9, (2018) Springer Nature: https://doi.org/10.1038/s41467-017-02473-5.en_US
dc.identifier.doihttps://doi.org/10.1038/s41467-017-02473-5en_US
dc.identifier.urihttps://hdl.handle.net/1911/102335en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the articleﾒs Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the articleﾒs Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleTuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factorsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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