High frame rate video mosaicking microendoscope to image large regions of intact tissue with subcellular resolution

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dc.citation.firstpage2800
dc.citation.issueNumber5
dc.citation.journalTitleBiomedical Optics Express
dc.citation.lastpage2812
dc.citation.volumeNumber12
dc.contributor.authorHunt, Brady
dc.contributor.authorCoole, Jackson
dc.contributor.authorBrenes, David
dc.contributor.authorKortum, Alex
dc.contributor.authorMitbander, Ruchika
dc.contributor.authorVohra, Imran
dc.contributor.authorCarns, Jennifer
dc.contributor.authorSchwarz, Richard
dc.contributor.authorRichards-Kortum, Rebecca
dc.date.accessioned2021-06-07T20:22:37Z
dc.date.available2021-06-07T20:22:37Z
dc.date.issued2021
dc.description.abstractHigh-resolution microendoscopy (HRME) is a low-cost strategy to acquire images of intact tissue with subcellular resolution at frame rates ranging from 11 to 18 fps. Current HRME imaging strategies are limited by the small microendoscope field of view (∼0.5 mm2); multiple images must be acquired and reliably registered to assess large regions of clinical interest. Image mosaics have been assembled from co-registered frames of video acquired as a microendoscope is slowly moved across the tissue surface, but the slow frame rate of previous HRME systems made this approach impractical for acquiring quality mosaicked images from large regions of interest. Here, we present a novel video mosaicking microendoscope incorporating a high frame rate CMOS sensor and optical probe holder to enable high-speed, high quality interrogation of large tissue regions of interest. Microendoscopy videos acquired at >90 fps are assembled into an image mosaic. We assessed registration accuracy and image sharpness across the mosaic for images acquired with a handheld probe over a range of translational speeds. This high frame rate video mosaicking microendoscope enables in vivo probe translation at >15 millimeters per second while preserving high image quality and accurate mosaicking, increasing the size of the region of interest that can be interrogated at high resolution from 0.5 mm2 to >30 mm2. Real-time deployment of this high-frame rate system is demonstrated in vivo and source code made publicly available.
dc.identifier.citationHunt, Brady, Coole, Jackson, Brenes, David, et al.. "High frame rate video mosaicking microendoscope to image large regions of intact tissue with subcellular resolution." <i>Biomedical Optics Express,</i> 12, no. 5 (2021) Optical Society of America: 2800-2812. https://doi.org/10.1364/BOE.425527.
dc.identifier.digitalboe-12-5-2800
dc.identifier.doihttps://doi.org/10.1364/BOE.425527
dc.identifier.urihttps://hdl.handle.net/1911/110684
dc.language.isoeng
dc.publisherOptical Society of America
dc.rightsPublished under the terms of the OSA Open Access Publishing Agreement
dc.rights.urihttps://www.osapublishing.org/library/license_v1.cfm#VOR-OA
dc.titleHigh frame rate video mosaicking microendoscope to image large regions of intact tissue with subcellular resolution
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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