High frame rate video mosaicking microendoscope to image large regions of intact tissue with subcellular resolution

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dc.citation.firstpage2800en_US
dc.citation.issueNumber5en_US
dc.citation.journalTitleBiomedical Optics Expressen_US
dc.citation.lastpage2812en_US
dc.citation.volumeNumber12en_US
dc.contributor.authorHunt, Bradyen_US
dc.contributor.authorCoole, Jacksonen_US
dc.contributor.authorBrenes, Daviden_US
dc.contributor.authorKortum, Alexen_US
dc.contributor.authorMitbander, Ruchikaen_US
dc.contributor.authorVohra, Imranen_US
dc.contributor.authorCarns, Jenniferen_US
dc.contributor.authorSchwarz, Richarden_US
dc.contributor.authorRichards-Kortum, Rebeccaen_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2021-06-07T20:22:37Zen_US
dc.date.available2021-06-07T20:22:37Zen_US
dc.date.issued2021en_US
dc.description.abstractHigh-resolution microendoscopy (HRME) is a low-cost strategy to acquire images of intact tissue with subcellular resolution at frame rates ranging from 11 to 18 fps. Current HRME imaging strategies are limited by the small microendoscope field of view (∼0.5 mm2); multiple images must be acquired and reliably registered to assess large regions of clinical interest. Image mosaics have been assembled from co-registered frames of video acquired as a microendoscope is slowly moved across the tissue surface, but the slow frame rate of previous HRME systems made this approach impractical for acquiring quality mosaicked images from large regions of interest. Here, we present a novel video mosaicking microendoscope incorporating a high frame rate CMOS sensor and optical probe holder to enable high-speed, high quality interrogation of large tissue regions of interest. Microendoscopy videos acquired at >90 fps are assembled into an image mosaic. We assessed registration accuracy and image sharpness across the mosaic for images acquired with a handheld probe over a range of translational speeds. This high frame rate video mosaicking microendoscope enables in vivo probe translation at >15 millimeters per second while preserving high image quality and accurate mosaicking, increasing the size of the region of interest that can be interrogated at high resolution from 0.5 mm2 to >30 mm2. Real-time deployment of this high-frame rate system is demonstrated in vivo and source code made publicly available.en_US
dc.identifier.citationHunt, Brady, Coole, Jackson, Brenes, David, et al.. "High frame rate video mosaicking microendoscope to image large regions of intact tissue with subcellular resolution." <i>Biomedical Optics Express,</i> 12, no. 5 (2021) Optical Society of America: 2800-2812. https://doi.org/10.1364/BOE.425527.en_US
dc.identifier.digitalboe-12-5-2800en_US
dc.identifier.doihttps://doi.org/10.1364/BOE.425527en_US
dc.identifier.urihttps://hdl.handle.net/1911/110684en_US
dc.language.isoengen_US
dc.publisherOptical Society of Americaen_US
dc.rightsPublished under the terms of the OSA Open Access Publishing Agreementen_US
dc.rights.urihttps://www.osapublishing.org/library/license_v1.cfm#VOR-OAen_US
dc.titleHigh frame rate video mosaicking microendoscope to image large regions of intact tissue with subcellular resolutionen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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