Identifying Behavioral Phenotypes and Heterogeneity in Heart Valve Surface Endothelium

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dc.citation.firstpage268en_US
dc.citation.issueNumber4en_US
dc.citation.journalTitleCells Tissues Organsen_US
dc.citation.lastpage276en_US
dc.citation.volumeNumber201en_US
dc.contributor.authorBlancas, Alicia A.en_US
dc.contributor.authorBalaoing, Liezl R.en_US
dc.contributor.authorAcosta, Francisca M.en_US
dc.contributor.authorGrande-Allen, K. Janeen_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2017-05-05T19:00:54Zen_US
dc.date.available2017-05-05T19:00:54Zen_US
dc.date.issued2016en_US
dc.description.abstractHeart valvular endothelial cells (VECs) are distinct from vascular endothelial cells (ECs), but have an uncertain context within the spectrum of known endothelial phenotypes, including lymphatic ECs (LECs). Profiling the phenotypes of the heart valve surface VECs would facilitate identification of a proper seeding population for tissue-engineered valves, as well as elucidate mechanisms of valvular disease. Porcine VECs and porcine aortic ECs (AECs) were isolated from pig hearts and characterized to assess known EC and LEC markers. A transwell migration assay determined their propensity to migrate toward vascular endothelial growth factor, an angiogenic stimulus, over 24 h. Compared to AECs, Flt-1 was expressed on almost double the percentage of VECs, measured as 74 versus 38%. The expression of angiogenic EC markers CXCR4 and DLL4 was >90% on AECs, whereas VECs showed only 35% CXCR4+ and 47% DLL4+. AECs demonstrated greater migration (71.5 ± 11.0 cells per image field) than the VECs with 30.0 ± 15.3 cells per image field (p = 0.032). In total, 30% of VECs were positive for LYVE1+/Prox1+, while these markers were absent in AECs. In conclusion, the population of cells on the surface of heart valves is heterogeneous, consisting largely of nonangiogenic VECs and a subset of LECs. Previous studies have indicated the presence of LECs within the interior of the valves; however, this is the first study to demonstrate their presence on the surface. Identification of this unique endothelial mixture is a step forward in the development of engineered valve replacements as a uniform EC seeding population may not be the best option to maximize transplant success.en_US
dc.identifier.citationBlancas, Alicia A., Balaoing, Liezl R., Acosta, Francisca M., et al.. "Identifying Behavioral Phenotypes and Heterogeneity in Heart Valve Surface Endothelium." <i>Cells Tissues Organs,</i> 201, no. 4 (2016) Karger: 268-276. https://doi.org/10.1159/000444446.en_US
dc.identifier.doihttps://doi.org/10.1159/000444446en_US
dc.identifier.urihttps://hdl.handle.net/1911/94201en_US
dc.language.isoengen_US
dc.publisherKargeren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Karger.en_US
dc.subject.keywordendotheliumen_US
dc.subject.keywordaortic valveen_US
dc.subject.keywordlymphaticen_US
dc.subject.keywordangiogenesisen_US
dc.titleIdentifying Behavioral Phenotypes and Heterogeneity in Heart Valve Surface Endotheliumen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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