A targeted CRISPR-Cas9 mediated F0 screen identifies genes involved in establishment of the enteric nervous system
| dc.citation.articleNumber | e0303914 | en_US |
| dc.citation.issueNumber | 5 | en_US |
| dc.citation.journalTitle | PLOS ONE | en_US |
| dc.citation.volumeNumber | 19 | en_US |
| dc.contributor.author | Moreno-Campos, Rodrigo | en_US |
| dc.contributor.author | Singleton, Eileen W. | en_US |
| dc.contributor.author | Uribe, Rosa A. | en_US |
| dc.contributor.org | Laboratory of Neural Crest and Enteric Nervous System Development | en_US |
| dc.date.accessioned | 2024-08-02T13:32:09Z | en_US |
| dc.date.available | 2024-08-02T13:32:09Z | en_US |
| dc.date.issued | 2024 | en_US |
| dc.description.abstract | The vertebrate enteric nervous system (ENS) is a crucial network of enteric neurons and glia resident within the entire gastrointestinal tract (GI). Overseeing essential GI functions such as gut motility and water balance, the ENS serves as a pivotal bidirectional link in the gut-brain axis. During early development, the ENS is primarily derived from enteric neural crest cells (ENCCs). Disruptions to ENCC development, as seen in conditions like Hirschsprung disease (HSCR), lead to the absence of ENS in the GI, particularly in the colon. In this study, using zebrafish, we devised an in vivo F0 CRISPR-based screen employing a robust, rapid pipeline integrating single-cell RNA sequencing, CRISPR reverse genetics, and high-content imaging. Our findings unveil various genes, including those encoding opioid receptors, as possible regulators of ENS establishment. In addition, we present evidence that suggests opioid receptor involvement in the neurochemical coding of the larval ENS. In summary, our work presents a novel, efficient CRISPR screen targeting ENS development, facilitating the discovery of previously unknown genes, and increasing knowledge of nervous system construction. | en_US |
| dc.identifier.citation | Moreno-Campos, R., Singleton, E. W., & Uribe, R. A. (2024). A targeted CRISPR-Cas9 mediated F0 screen identifies genes involved in establishment of the enteric nervous system. PLOS ONE, 19(5), e0303914. https://doi.org/10.1371/journal.pone.0303914 | en_US |
| dc.identifier.digital | journal-pone-0303914 | en_US |
| dc.identifier.doi | https://doi.org/10.1371/journal.pone.0303914 | en_US |
| dc.identifier.uri | https://hdl.handle.net/1911/117573 | en_US |
| dc.language.iso | eng | en_US |
| dc.publisher | Public Library of Science | en_US |
| dc.rights | Except where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license. Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder. | en_US |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
| dc.title | A targeted CRISPR-Cas9 mediated F0 screen identifies genes involved in establishment of the enteric nervous system | en_US |
| dc.type | Journal article | en_US |
| dc.type.dcmi | Text | en_US |
| dc.type.publication | publisher version | en_US |
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