A Technique to Increase Accessibility to Late-Stage Chick Embryos for In Ovo Manipulations

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dc.citation.firstpage148
dc.citation.issueNumber2
dc.citation.journalTitleDevelopmental Dynamics
dc.citation.lastpage154
dc.citation.volumeNumber242
dc.contributor.authorSpurlin, James III
dc.contributor.authorLwigale, Peter Y.
dc.date.accessioned2014-12-15T20:35:10Z
dc.date.available2014-12-15T20:35:10Z
dc.date.issued2013
dc.description.abstractBackground: During early development, avian embryos are easily accessible in ovo for transplantations and experimental perturbations. However, these qualities of the avian embryonic model rapidly wane shortly after embryonic day (E)4 when the embryo is obscured by extraembryonic membranes, making it difficult to study developmental events that occur at later stages in vivo. Results: In this study, we describe a multistep method that involves initially windowing eggs at E3, followed by dissecting away extraembryonic membranes at E5 to facilitate embryo accessibility in ovo until later stages of development. The majority of the embryos subjected to this technique remain exposed between E5 and E8, then become gradually displaced by the growing allantois from posterior to anterior regions. Conclusions: Exposed embryos are viable and compatible with embryological and modern developmental biology techniques including tissue grafting and ablation, gene manipulation by electroporation, and protein expression. This technique opens up new avenues for studying complex cellular interactions during organogenesis and can be further extrapolated to regeneration and stem cell studies.
dc.identifier.citationSpurlin, James III and Lwigale, Peter Y.. "A Technique to Increase Accessibility to Late-Stage Chick Embryos for In Ovo Manipulations." <i>Developmental Dynamics,</i> 242, no. 2 (2013) Wiley: 148-154. http://dx.doi.org/10.1002/dvdy.23907.
dc.identifier.doihttp://dx.doi.org/10.1002/dvdy.23907
dc.identifier.urihttps://hdl.handle.net/1911/78759
dc.language.isoeng
dc.publisherWiley
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
dc.subject.keywordlate-stage chick embryo
dc.subject.keywordin ovo
dc.subject.keywordextraembryonic membranes
dc.subject.keyworddissection
dc.subject.keywordcell injections
dc.titleA Technique to Increase Accessibility to Late-Stage Chick Embryos for In Ovo Manipulations
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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