A Technique to Increase Accessibility to Late-Stage Chick Embryos for In Ovo Manipulations

dc.citation.firstpage148en_US
dc.citation.issueNumber2en_US
dc.citation.journalTitleDevelopmental Dynamicsen_US
dc.citation.lastpage154en_US
dc.citation.volumeNumber242en_US
dc.contributor.authorSpurlin, James IIIen_US
dc.contributor.authorLwigale, Peter Y.en_US
dc.date.accessioned2014-12-15T20:35:10Zen_US
dc.date.available2014-12-15T20:35:10Zen_US
dc.date.issued2013en_US
dc.description.abstractBackground: During early development, avian embryos are easily accessible in ovo for transplantations and experimental perturbations. However, these qualities of the avian embryonic model rapidly wane shortly after embryonic day (E)4 when the embryo is obscured by extraembryonic membranes, making it difficult to study developmental events that occur at later stages in vivo. Results: In this study, we describe a multistep method that involves initially windowing eggs at E3, followed by dissecting away extraembryonic membranes at E5 to facilitate embryo accessibility in ovo until later stages of development. The majority of the embryos subjected to this technique remain exposed between E5 and E8, then become gradually displaced by the growing allantois from posterior to anterior regions. Conclusions: Exposed embryos are viable and compatible with embryological and modern developmental biology techniques including tissue grafting and ablation, gene manipulation by electroporation, and protein expression. This technique opens up new avenues for studying complex cellular interactions during organogenesis and can be further extrapolated to regeneration and stem cell studies.en_US
dc.identifier.citationSpurlin, James III and Lwigale, Peter Y.. "A Technique to Increase Accessibility to Late-Stage Chick Embryos for In Ovo Manipulations." <i>Developmental Dynamics,</i> 242, no. 2 (2013) Wiley: 148-154. http://dx.doi.org/10.1002/dvdy.23907.en_US
dc.identifier.doihttp://dx.doi.org/10.1002/dvdy.23907en_US
dc.identifier.urihttps://hdl.handle.net/1911/78759en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.en_US
dc.subject.keywordlate-stage chick embryoen_US
dc.subject.keywordin ovoen_US
dc.subject.keywordextraembryonic membranesen_US
dc.subject.keyworddissectionen_US
dc.subject.keywordcell injectionsen_US
dc.titleA Technique to Increase Accessibility to Late-Stage Chick Embryos for In Ovo Manipulationsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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