EfgA is a conserved formaldehyde sensor that leads to bacterial growth arrest in response to elevated formaldehyde

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dc.citation.articleNumbere3001208en_US
dc.citation.issueNumber5en_US
dc.citation.journalTitlePLOS Biologyen_US
dc.citation.volumeNumber19en_US
dc.contributor.authorBazurto, Jannell V.en_US
dc.contributor.authorNayak, Dipti D.en_US
dc.contributor.authorTicak, Tomislaven_US
dc.contributor.authorDavlieva, Milyaen_US
dc.contributor.authorLee, Jessica A.en_US
dc.contributor.authorHellenbrand, Chandler N.en_US
dc.contributor.authorLambert, Leah B.en_US
dc.contributor.authorBenski, Olivia J.en_US
dc.contributor.authorQuates, Caleb J.en_US
dc.contributor.authorJohnson, Jill L.en_US
dc.contributor.authorPatel, Jagdish Sureshen_US
dc.contributor.authorYtreberg, F.Martyen_US
dc.contributor.authorShamoo, Yousifen_US
dc.contributor.authorMarx, Christopher J.en_US
dc.date.accessioned2021-06-10T17:36:55Zen_US
dc.date.available2021-06-10T17:36:55Zen_US
dc.date.issued2021en_US
dc.description.abstractNormal cellular processes give rise to toxic metabolites that cells must mitigate. Formaldehyde is a universal stressor and potent metabolic toxin that is generated in organisms from bacteria to humans. Methylotrophic bacteria such as Methylorubrum extorquens face an acute challenge due to their production of formaldehyde as an obligate central intermediate of single-carbon metabolism. Mechanisms to sense and respond to formaldehyde were speculated to exist in methylotrophs for decades but had never been discovered. Here, we identify a member of the DUF336 domain family, named efgA for enhanced formaldehyde growth, that plays an important role in endogenous formaldehyde stress response in M. extorquens PA1 and is found almost exclusively in methylotrophic taxa. Our experimental analyses reveal that EfgA is a formaldehyde sensor that rapidly arrests growth in response to elevated levels of formaldehyde. Heterologous expression of EfgA in Escherichia coli increases formaldehyde resistance, indicating that its interaction partners are widespread and conserved. EfgA represents the first example of a formaldehyde stress response system that does not involve enzymatic detoxification. Thus, EfgA comprises a unique stress response mechanism in bacteria, whereby a single protein directly senses elevated levels of a toxic intracellular metabolite and safeguards cells from potential damage.en_US
dc.identifier.citationBazurto, Jannell V., Nayak, Dipti D., Ticak, Tomislav, et al.. "EfgA is a conserved formaldehyde sensor that leads to bacterial growth arrest in response to elevated formaldehyde." <i>PLOS Biology,</i> 19, no. 5 (2021) Public Library of Science: https://doi.org/10.1371/journal.pbio.3001208.en_US
dc.identifier.digitaljournal-pbio-3001208en_US
dc.identifier.doihttps://doi.org/10.1371/journal.pbio.3001208en_US
dc.identifier.urihttps://hdl.handle.net/1911/110719en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleEfgA is a conserved formaldehyde sensor that leads to bacterial growth arrest in response to elevated formaldehydeen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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