A paper and plastic device for performing recombinase polymerase amplification of HIV DNA

Simple item page
dc.citation.firstpage3082
dc.citation.issueNumber17
dc.citation.journalTitleLab on a Chip
dc.citation.lastpage3088
dc.citation.volumeNumber12
dc.contributor.authorRohrman, Brittany A.
dc.contributor.authorRichards-Kortum, Rebecca R.
dc.date.accessioned2014-12-15T20:35:09Z
dc.date.available2014-12-15T20:35:09Z
dc.date.issued2012
dc.description.abstractDespite the importance of early diagnosis and treatment of HIV, only a small fraction of HIV-exposed infants in low- and middle-income countries are tested for the disease. The gold standard for early infant diagnosis, DNA PCR, requires resources that are unavailable in poor settings, and no point-of-care HIV DNA test is currently available. We have developed a device constructed of layers of paper, glass fiber, and plastic that is capable of performing isothermal, enzymatic amplification of HIV DNA. The device is inexpensive, small, light-weight, and easy to assemble. The device stores lyophilized enzymes, facilitates mixing of reaction components, and supports recombinase polymerase amplification in five steps of operation. Using commercially available lateral flow strips as a detection method, we demonstrate the ability of our device to amplify 10 copies of HIV DNA to detectable levels in 15 minutes. Our results suggest that our device, which is designed to be used after DNA extraction from dried-blood spots, may serve in conjunction with lateral flow strips as part of a point-of-care HIV DNA test to be used in low resource settings.
dc.identifier.citationRohrman, Brittany A. and Richards-Kortum, Rebecca R.. "A paper and plastic device for performing recombinase polymerase amplification of HIV DNA." <i>Lab on a Chip,</i> 12, no. 17 (2012) Royal Society of Chemistry: 3082-3088. http://dx.doi.org/10.1039/C2LC40423K.
dc.identifier.doihttp://dx.doi.org/10.1039/C2LC40423K
dc.identifier.urihttps://hdl.handle.net/1911/78755
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Royal Society of Chemistry
dc.titleA paper and plastic device for performing recombinase polymerase amplification of HIV DNA
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
nihms-437347.pdf
Size:
1.6 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications
Bioengineering Publications