Rapid casting of patterned vascular networks for perfusable engineered 3D tissues

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dc.citation.firstpage768en_US
dc.citation.issueNumber9en_US
dc.citation.journalTitleNature Materialsen_US
dc.citation.lastpage774en_US
dc.citation.volumeNumber11en_US
dc.contributor.authorMiller, Jordan S.en_US
dc.contributor.authorStevens, Kelly R.en_US
dc.contributor.authorYang, Michael T.en_US
dc.contributor.authorBaker, Brendon M.en_US
dc.contributor.authorNguyen, Duc-Huy T.en_US
dc.contributor.authorCohen, Daniel M.en_US
dc.contributor.authorToro, Estebanen_US
dc.contributor.authorChen, Alice A.en_US
dc.contributor.authorGalie, Peter A.en_US
dc.contributor.authorYu, Xiangen_US
dc.contributor.authorChaturvedi, Ritikaen_US
dc.contributor.authorBhatia, Sangeeta N.en_US
dc.contributor.authorChen, Christopher S.en_US
dc.date.accessioned2014-09-22T20:23:28Zen_US
dc.date.available2014-09-22T20:23:28Zen_US
dc.date.issued2012en_US
dc.description.abstractIn the absence of perfusable vascular networks, three-dimensional (3D) engineered tissues densely populated with cells quickly develop a necrotic core [1]. Yet the lack of a general approach to rapidly construct such networks remains a major challenge for 3D tissue culture [2-4]. Here, we 3D printed rigid filament networks of carbohydrate glass, and used them as a cytocompatible sacrificial template in engineered tissues containing living cells to generate cylindrical networks which could be lined with endothelial cells and perfused with blood under high-pressure pulsatile flow. Because this simple vascular casting approach allows independent control of network geometry, endothelialization, and extravascular tissue, it is compatible with a wide variety of cell types, synthetic and natural extracellular matrices (ECMs), and crosslinking strategies. We also demonstrated that the perfused vascular channels sustained the metabolic function of primary rat hepatocytes in engineered tissue constructs that otherwise exhibited suppressed function in their core.en_US
dc.identifier.citationMiller, Jordan S., Stevens, Kelly R., Yang, Michael T., et al.. "Rapid casting of patterned vascular networks for perfusable engineered 3D tissues." <i>Nature Materials,</i> 11, no. 9 (2012) Nature Publishing Group: 768-774. http://dx.doi.org/10.1038/nmat3357.en_US
dc.identifier.doihttp://dx.doi.org/10.1038/nmat3357en_US
dc.identifier.urihttps://hdl.handle.net/1911/77220en_US
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Nature Publishing Group.en_US
dc.titleRapid casting of patterned vascular networks for perfusable engineered 3D tissuesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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