Rapid casting of patterned vascular networks for perfusable engineered 3D tissues

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dc.citation.firstpage768
dc.citation.issueNumber9
dc.citation.journalTitleNature Materials
dc.citation.lastpage774
dc.citation.volumeNumber11
dc.contributor.authorMiller, Jordan S.
dc.contributor.authorStevens, Kelly R.
dc.contributor.authorYang, Michael T.
dc.contributor.authorBaker, Brendon M.
dc.contributor.authorNguyen, Duc-Huy T.
dc.contributor.authorCohen, Daniel M.
dc.contributor.authorToro, Esteban
dc.contributor.authorChen, Alice A.
dc.contributor.authorGalie, Peter A.
dc.contributor.authorYu, Xiang
dc.contributor.authorChaturvedi, Ritika
dc.contributor.authorBhatia, Sangeeta N.
dc.contributor.authorChen, Christopher S.
dc.date.accessioned2014-09-22T20:23:28Z
dc.date.available2014-09-22T20:23:28Z
dc.date.issued2012
dc.description.abstractIn the absence of perfusable vascular networks, three-dimensional (3D) engineered tissues densely populated with cells quickly develop a necrotic core [1]. Yet the lack of a general approach to rapidly construct such networks remains a major challenge for 3D tissue culture [2-4]. Here, we 3D printed rigid filament networks of carbohydrate glass, and used them as a cytocompatible sacrificial template in engineered tissues containing living cells to generate cylindrical networks which could be lined with endothelial cells and perfused with blood under high-pressure pulsatile flow. Because this simple vascular casting approach allows independent control of network geometry, endothelialization, and extravascular tissue, it is compatible with a wide variety of cell types, synthetic and natural extracellular matrices (ECMs), and crosslinking strategies. We also demonstrated that the perfused vascular channels sustained the metabolic function of primary rat hepatocytes in engineered tissue constructs that otherwise exhibited suppressed function in their core.
dc.identifier.citationMiller, Jordan S., Stevens, Kelly R., Yang, Michael T., et al.. "Rapid casting of patterned vascular networks for perfusable engineered 3D tissues." <i>Nature Materials,</i> 11, no. 9 (2012) Nature Publishing Group: 768-774. http://dx.doi.org/10.1038/nmat3357.
dc.identifier.doihttp://dx.doi.org/10.1038/nmat3357
dc.identifier.urihttps://hdl.handle.net/1911/77220
dc.language.isoeng
dc.publisherNature Publishing Group
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Nature Publishing Group.
dc.titleRapid casting of patterned vascular networks for perfusable engineered 3D tissues
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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