DNA Helicase–Polymerase Coupling in Bacteriophage DNA Replication

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dc.citation.articleNumber1739en_US
dc.citation.issueNumber9en_US
dc.citation.journalTitleVirusesen_US
dc.citation.volumeNumber13en_US
dc.contributor.authorLo, Chen-Yuen_US
dc.contributor.authorGao, Yangen_US
dc.date.accessioned2021-09-21T15:37:57Zen_US
dc.date.available2021-09-21T15:37:57Zen_US
dc.date.issued2021en_US
dc.description.abstractBacteriophages have long been model systems to study the molecular mechanisms of DNA replication. During DNA replication, a DNA helicase and a DNA polymerase cooperatively unwind the parental DNA. By surveying recent data from three bacteriophage replication systems, we summarized the mechanistic basis of DNA replication by helicases and polymerases. Kinetic data have suggested that a polymerase or a helicase alone is a passive motor that is sensitive to the base-pairing energy of the DNA. When coupled together, the helicase–polymerase complex is able to unwind DNA actively. In bacteriophage T7, helicase and polymerase reside right at the replication fork where the parental DNA is separated into two daughter strands. The two motors pull the two daughter strands to opposite directions, while the polymerase provides a separation pin to split the fork. Although independently evolved and containing different replisome components, bacteriophage T4 replisome shares mechanistic features of Hel–Pol coupling that are similar to T7. Interestingly, in bacteriophages with a limited size of genome like Φ29, DNA polymerase itself can form a tunnel-like structure, which encircles the DNA template strand and facilitates strand displacement synthesis in the absence of a helicase. Studies on bacteriophage replication provide implications for the more complicated replication systems in bacteria, archaeal, and eukaryotic systems, as well as the RNA genome replication in RNA viruses.en_US
dc.identifier.citationLo, Chen-Yu and Gao, Yang. "DNA Helicase–Polymerase Coupling in Bacteriophage DNA Replication." <i>Viruses,</i> 13, no. 9 (2021) MDPI: https://doi.org/10.3390/v13091739.en_US
dc.identifier.digitalviruses-13-01739-v2en_US
dc.identifier.doihttps://doi.org/10.3390/v13091739en_US
dc.identifier.urihttps://hdl.handle.net/1911/111397en_US
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citeden_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleDNA Helicase–Polymerase Coupling in Bacteriophage DNA Replicationen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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