Effects of cellular parameters on the in vitro osteogenic potential of dual-gelling mesenchymal stem cell-laden hydrogels

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dc.citation.firstpage1277en_US
dc.citation.issueNumber12en_US
dc.citation.journalTitleJournal of Biomaterials Science, Polymer Editionen_US
dc.citation.lastpage1290en_US
dc.citation.volumeNumber27en_US
dc.contributor.authorVo, Tiffany N.en_US
dc.contributor.authorTabata, Yasuhikoen_US
dc.contributor.authorMikos, Antonios G.en_US
dc.date.accessioned2017-08-09T17:13:26Zen_US
dc.date.available2017-08-09T17:13:26Zen_US
dc.date.issued2016en_US
dc.description.abstractThis work investigated the effects of cellular encapsulation density and differentiation stage on the osteogenic capacity of injectable, dual physically and chemically gelling hydrogels comprised of thermogelling macromers and polyamidoamine crosslinkers. Undifferentiated and osteogenically predifferentiated mesenchymal stem cells (MSCs) were encapsulated within 20 wt% composite hydrogels with gelatin microparticles at densities of six or 15 million cells/mL. We hypothesized that a high encapsulation density and predifferentiation would promote increased cellular interaction and accelerate osteogenesis, leading to enhanced osteogenic potential in vitro. Hydrogels were able to maintain the viability of the encapsulated cells over a period of 28 days, with the high encapsulation density and predifferentiation group possessing the highest DNA content at all time points. Early alkaline phosphatase activity and mineralization were promoted by encapsulation density, whereas this effect by predifferentiation was only observed in the low seeding density groups. Both parameters only demonstrated short-lived effects when examined independently, but jointly led to greater levels of alkaline phosphatase activity and mineralization. The combined effects suggest that there may be optimal encapsulation densities and differentiation periods that need to be investigated to improve MSCs for biomaterial-based therapeutics in bone tissue engineering.en_US
dc.identifier.citationVo, Tiffany N., Tabata, Yasuhiko and Mikos, Antonios G.. "Effects of cellular parameters on the in vitro osteogenic potential of dual-gelling mesenchymal stem cell-laden hydrogels." <i>Journal of Biomaterials Science, Polymer Edition,</i> 27, no. 12 (2016) Taylor & Francis: 1277-1290. http://dx.doi.org/10.1080/09205063.2016.1195157.en_US
dc.identifier.digitalEffects_Cellular_Parametersen_US
dc.identifier.doihttp://dx.doi.org/10.1080/09205063.2016.1195157en_US
dc.identifier.urihttps://hdl.handle.net/1911/96633en_US
dc.language.isoengen_US
dc.publisherTaylor & Francisen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Taylor & Francis.en_US
dc.subject.keywordMesenchymal stem cellen_US
dc.subject.keywordN-isopropylacrylamideen_US
dc.subject.keywordbone tissue engineeringen_US
dc.subject.keywordpredifferentiationen_US
dc.titleEffects of cellular parameters on the in vitro osteogenic potential of dual-gelling mesenchymal stem cell-laden hydrogelsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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