Amniotic fluid-derived stem cells demonstrate limited cardiac differentiation following small molecule-based modulation of Wnt signaling pathway

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dc.citation.firstpage34103en_US
dc.citation.issueNumber3en_US
dc.citation.journalTitleBiomedical Materialsen_US
dc.citation.volumeNumber10en_US
dc.contributor.authorConnell, Jennifer Petscheen_US
dc.contributor.authorRuano, Rodrigoen_US
dc.contributor.authorJacot, Jeffrey G.en_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2015-06-24T18:59:27Zen_US
dc.date.available2015-06-24T18:59:27Zen_US
dc.date.issued2015en_US
dc.description.abstractAmniotic fluid-derived stem cells (AFSC) are a promising cell source for regenerative medicine and cardiac tissue engineering. However, a non-xenotropic differentiation protocol has not been established for cardiac differentiation of AFSC. We tested a small molecule-based modulation of Wnt signaling for directed cardiac differentiation of AFSC. Cells were treated with inhibitors of glycogen synthase kinase 3 and Wnt production and secretion in a time-dependent and sequential manner, as has been demonstrated successful for cardiac differentiation of embryonic and induced pluripotent stem cells. Cells were then analyzed for gene and protein expression of markers along the cardiac lineage at multiple days during the differentiation protocol. At the midpoint of the differentiation, an increase in the percentage of AFSC expressing Islet-1, a transcription factor found in cardiac progenitor cells, and Nkx-2.5, a cardiac transcription factor, was observed. After a 15 d differentiation, a subpopulation of AFSC upregulated protein expression of smooth muscle actin, myosin light chain-2, and troponin I, all indicative of progression down a cardiac lineage. AFSC at the end of the differentiation also demonstrated organization of connexin 43, a key component of gap junctions, to cell membranes. However, no organized sarcomeres or spontaneous contraction were observed. These results demonstrate that small molecule-based modulation of Wnt signaling alone is not sufficient to generate functional cardiomyocytes from AFSC, though an upregulation of genes and proteins common to cardiac lineage cells was observed.en_US
dc.identifier.citationConnell, Jennifer Petsche, Ruano, Rodrigo and Jacot, Jeffrey G.. "Amniotic fluid-derived stem cells demonstrate limited cardiac differentiation following small molecule-based modulation of Wnt signaling pathway." <i>Biomedical Materials,</i> 10, no. 3 (2015) IOP Publishing: 34103. http://dx.doi.org/10.1088/1748-6041/10/3/034103.en_US
dc.identifier.doihttp://dx.doi.org/10.1088/1748-6041/10/3/034103en_US
dc.identifier.urihttps://hdl.handle.net/1911/80771en_US
dc.language.isoengen_US
dc.publisherIOP Publishingen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by IOP Publishingen_US
dc.titleAmniotic fluid-derived stem cells demonstrate limited cardiac differentiation following small molecule-based modulation of Wnt signaling pathwayen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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