Super-Resolution mbPAINT for Optical Localization of Single-Stranded DNA

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dc.citation.firstpage9338
dc.citation.issueNumber19
dc.citation.journalTitleACS Applied Materials & Interfaces
dc.citation.lastpage9343
dc.citation.volumeNumber5
dc.contributor.authorChen, Jixin
dc.contributor.authorBremauntz, Alberto
dc.contributor.authorKisley, Lydia
dc.contributor.authorShuang, Bo
dc.contributor.authorLandes, Christy F.
dc.date.accessioned2015-07-10T18:42:19Z
dc.date.available2015-07-10T18:42:19Z
dc.date.issued2013
dc.description.abstractWe demonstrate the application of superlocalization microscopy to identify sequence-specific portions of single-stranded DNA (ssDNA) with sequence resolution of 50 nucleotides, corresponding to a spatial resolution of 30 nm. Super-resolution imaging was achieved using a variation of a single-molecule localization method, termed as ﾓmotion blurﾔ point accumulation for imaging in nanoscale topography (mbPAINT). The target ssDNA molecules were immobilized on the substrate. Short, dye-labeled, and complementary ssDNA molecules stochastically bound to the target ssDNA, with repeated binding events allowing super-resolution. Sequence specificity was demonstrated via the use of a control, noncomplementary probe. The results support the possibility of employing relatively inexpensive short ssDNAs to identify gene sequence specificity with improved resolution in comparison to the existing methods.
dc.identifier.citationChen, Jixin, Bremauntz, Alberto, Kisley, Lydia, et al.. "Super-Resolution mbPAINT for Optical Localization of Single-Stranded DNA." <i>ACS Applied Materials & Interfaces,</i> 5, no. 19 (2013) American Chemical Society: 9338-9343. http://dx.doi.org/10.1021/am403984k.
dc.identifier.doihttp://dx.doi.org/10.1021/am403984k
dc.identifier.urihttps://hdl.handle.net/1911/80888
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.
dc.subject.keywordsuper-resolution microscopy
dc.subject.keywordsingle-stranded DNA
dc.subject.keywordoptical mapping
dc.subject.keywordmbPAINT
dc.subject.keywordbiosensing
dc.subject.keywordmaterials and biointerfaces
dc.titleSuper-Resolution mbPAINT for Optical Localization of Single-Stranded DNA
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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