Super-Resolution mbPAINT for Optical Localization of Single-Stranded DNA

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dc.citation.firstpage9338en_US
dc.citation.issueNumber19en_US
dc.citation.journalTitleACS Applied Materials & Interfacesen_US
dc.citation.lastpage9343en_US
dc.citation.volumeNumber5en_US
dc.contributor.authorChen, Jixinen_US
dc.contributor.authorBremauntz, Albertoen_US
dc.contributor.authorKisley, Lydiaen_US
dc.contributor.authorShuang, Boen_US
dc.contributor.authorLandes, Christy F.en_US
dc.date.accessioned2015-07-10T18:42:19Zen_US
dc.date.available2015-07-10T18:42:19Zen_US
dc.date.issued2013en_US
dc.description.abstractWe demonstrate the application of superlocalization microscopy to identify sequence-specific portions of single-stranded DNA (ssDNA) with sequence resolution of 50 nucleotides, corresponding to a spatial resolution of 30 nm. Super-resolution imaging was achieved using a variation of a single-molecule localization method, termed as ﾓmotion blurﾔ point accumulation for imaging in nanoscale topography (mbPAINT). The target ssDNA molecules were immobilized on the substrate. Short, dye-labeled, and complementary ssDNA molecules stochastically bound to the target ssDNA, with repeated binding events allowing super-resolution. Sequence specificity was demonstrated via the use of a control, noncomplementary probe. The results support the possibility of employing relatively inexpensive short ssDNAs to identify gene sequence specificity with improved resolution in comparison to the existing methods.en_US
dc.identifier.citationChen, Jixin, Bremauntz, Alberto, Kisley, Lydia, et al.. "Super-Resolution mbPAINT for Optical Localization of Single-Stranded DNA." <i>ACS Applied Materials & Interfaces,</i> 5, no. 19 (2013) American Chemical Society: 9338-9343. http://dx.doi.org/10.1021/am403984k.en_US
dc.identifier.doihttp://dx.doi.org/10.1021/am403984ken_US
dc.identifier.urihttps://hdl.handle.net/1911/80888en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.en_US
dc.subject.keywordsuper-resolution microscopyen_US
dc.subject.keywordsingle-stranded DNAen_US
dc.subject.keywordoptical mappingen_US
dc.subject.keywordmbPAINTen_US
dc.subject.keywordbiosensingen_US
dc.subject.keywordmaterials and biointerfacesen_US
dc.titleSuper-Resolution mbPAINT for Optical Localization of Single-Stranded DNAen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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