Structure of RNA 3'-phosphate cyclase bound to substrate RNA
| dc.citation.journalTitle | RNA | en_US |
| dc.contributor.author | Desai, Kevin K. | en_US |
| dc.contributor.author | Bingman, Craig A. | en_US |
| dc.contributor.author | Cheng, Chin L. | en_US |
| dc.contributor.author | Phillips, George N. Jr. | en_US |
| dc.contributor.author | Raines, Ronald T. | en_US |
| dc.date.accessioned | 2014-09-30T16:31:17Z | en_US |
| dc.date.available | 2014-09-30T16:31:17Z | en_US |
| dc.date.issued | 2014 | en_US |
| dc.description.abstract | RNA 3′-phosphate cyclase (RtcA) catalyzes the ATP-dependent cyclization of a 3′-phosphate to form a 2′,3′-cyclic phosphate at RNA termini. Cyclization proceeds through RtcA–AMP and RNA(3′)pp(5′)A covalent intermediates, which are analogous to intermediates formed during catalysis by the tRNA ligase RtcB. Here we present a crystal structure of Pyrococcus horikoshii RtcA in complex with a 3′-phosphate terminated RNA and adenosine in the AMP-binding pocket. Our data reveal that RtcA recognizes substrate RNA by ensuring that the terminal 3′-phosphate makes a large contribution to RNA binding. Furthermore, the RNA 3′-phosphate is poised for in-line attack on the P–N bond that links the phosphorous atom of AMP to Nε of His307. Thus, we provide the first insights into RNA 3′-phosphate termini recognition and the mechanism of 3′-phosphate activation by an Rtc enzyme. | en_US |
| dc.identifier.citation | Desai, Kevin K., Bingman, Craig A., Cheng, Chin L., et al.. "Structure of RNA 3'-phosphate cyclase bound to substrate RNA." <i>RNA,</i> (2014) Cold Spring Harbor Laboratory Press: http://dx.doi.org/10.1261/rna.045823.114. | en_US |
| dc.identifier.doi | http://dx.doi.org/10.1261/rna.045823.114 | en_US |
| dc.identifier.uri | https://hdl.handle.net/1911/77319 | en_US |
| dc.language.iso | eng | en_US |
| dc.publisher | Cold Spring Harbor Laboratory Press | en_US |
| dc.rights | Available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ | en_US |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | en_US |
| dc.subject.keyword | RtcA | en_US |
| dc.subject.keyword | RNA 3'-phosphate termini | en_US |
| dc.subject.keyword | 2',3'-cyclic phosphate termini | en_US |
| dc.title | Structure of RNA 3'-phosphate cyclase bound to substrate RNA | en_US |
| dc.type | Journal article | en_US |
| dc.type.dcmi | Text | en_US |
| dc.type.publication | publisher version | en_US |
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