Structure of RNA 3'-phosphate cyclase bound to substrate RNA

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dc.citation.journalTitleRNA
dc.contributor.authorDesai, Kevin K.
dc.contributor.authorBingman, Craig A.
dc.contributor.authorCheng, Chin L.
dc.contributor.authorPhillips, George N. Jr.
dc.contributor.authorRaines, Ronald T.
dc.date.accessioned2014-09-30T16:31:17Z
dc.date.available2014-09-30T16:31:17Z
dc.date.issued2014
dc.description.abstractRNA 3′-phosphate cyclase (RtcA) catalyzes the ATP-dependent cyclization of a 3′-phosphate to form a 2′,3′-cyclic phosphate at RNA termini. Cyclization proceeds through RtcA–AMP and RNA(3′)pp(5′)A covalent intermediates, which are analogous to intermediates formed during catalysis by the tRNA ligase RtcB. Here we present a crystal structure of Pyrococcus horikoshii RtcA in complex with a 3′-phosphate terminated RNA and adenosine in the AMP-binding pocket. Our data reveal that RtcA recognizes substrate RNA by ensuring that the terminal 3′-phosphate makes a large contribution to RNA binding. Furthermore, the RNA 3′-phosphate is poised for in-line attack on the P–N bond that links the phosphorous atom of AMP to Nε of His307. Thus, we provide the first insights into RNA 3′-phosphate termini recognition and the mechanism of 3′-phosphate activation by an Rtc enzyme.
dc.identifier.citationDesai, Kevin K., Bingman, Craig A., Cheng, Chin L., et al.. "Structure of RNA 3'-phosphate cyclase bound to substrate RNA." <i>RNA,</i> (2014) Cold Spring Harbor Laboratory Press: http://dx.doi.org/10.1261/rna.045823.114.
dc.identifier.doihttp://dx.doi.org/10.1261/rna.045823.114
dc.identifier.urihttps://hdl.handle.net/1911/77319
dc.language.isoeng
dc.publisherCold Spring Harbor Laboratory Press
dc.rightsAvailable under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subject.keywordRtcA
dc.subject.keywordRNA 3'-phosphate termini
dc.subject.keyword2',3'-cyclic phosphate termini
dc.titleStructure of RNA 3'-phosphate cyclase bound to substrate RNA
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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