Emotion polyregulation is an essential aspect of responding to stressful events by employing multiple strategies to influence one’s emotions. However, the specific patterns of emotion polyregulation that effectively mitigate negative affect remain largely unknown. I attempted to identify adaptive profiles of emotion polyregulation and its effectiveness compared to general strategy variability in a series of five studies. This research investigates global reports of emotion polyregulation and their association with high-stress adult populations in real-word settings, such as bereaved spouses and individuals quarantining during the COVID-19 pandemic (Studies 1-2; Aim A). Additionally, this research examines daily momentary reports of emotion polyregulation in a university sample and explores how they are influenced by cognitive, psychological, and physiological individual differences (Study 3; Aim B). Furthermore, this study reviews the utilization of emotion polyregulation in current psychointerventions for clinically relevant populations, specifically family caregivers for individuals with Alzheimer's Disease and related dementias. It also introduces an emotion polyregulation training paradigm to investigate the impact of cognitive reappraisal and its tactics on the success of emotion polyregulation (Studies 4-5; Aim C). The findings of these studies provide insights into successful patterns of emotion polyregulation and compare the impact of different polyregulation profiles on negative affect, in contrast to general strategy variability.