Structural Basis of Actin Filament Nucleation by Tandem W Domains

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dc.citation.firstpage1
dc.citation.journalTitleCell Reports
dc.citation.lastpage11
dc.citation.volumeNumber3
dc.contributor.authorChen, Xiaorui
dc.contributor.authorNi, Fengyun
dc.contributor.authorTian, Xia
dc.contributor.authorKondrashkina, Elena
dc.contributor.authorWang, Qinghua
dc.contributor.authorMa, Jianpeng
dc.date.accessioned2013-06-19T16:46:14Z
dc.date.available2013-06-19T16:46:14Z
dc.date.issued2013
dc.description.abstractSpontaneous nucleation of actin is very inefficient in cells. To overcome this barrier, cells have evolved a set of actin filament nucleators to promote rapid nucleation and polymerization in response to specific stimuli. However, the molecular mechanism of actin nucleation remains poorly understood. This is hindered largely by the fact that actin nucleus, once formed, rapidly polymerizes into filament, thus making it impossible to capture stable multisubunit actin nucleus. Here, we report an effective doublemutant strategy to stabilize actin nucleus by preventing further polymerization. Employing this strategy, we solved the crystal structure of AMPPNP-actin in complex with the first two tandem W domains of Cordon-bleu (Cobl), a potent actin filament nucleator. Further sequence comparison and functional studies suggest that the nucleation mechanism of Cobl is probably shared by the p53 cofactor JMY, but not Spire. Moreover, the double-mutant strategy opens the way for atomic mechanistic study of actin nucleation and polymerization.
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dc.identifier.citationChen, Xiaorui, Ni, Fengyun, Tian, Xia, et al.. "Structural Basis of Actin Filament Nucleation by Tandem W Domains." <i>Cell Reports,</i> 3, (2013) Cell Press: 1-11. http://dx.doi.org/10.1016/j.celrep.2013.04.028.
dc.identifier.doihttp://dx.doi.org/10.1016/j.celrep.2013.04.028
dc.identifier.urihttps://hdl.handle.net/1911/71326
dc.language.isoeng
dc.publisherCell Press
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.titleStructural Basis of Actin Filament Nucleation by Tandem W Domains
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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