Structural Basis of Actin Filament Nucleation by Tandem W Domains

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dc.citation.firstpage1en_US
dc.citation.journalTitleCell Reportsen_US
dc.citation.lastpage11en_US
dc.citation.volumeNumber3en_US
dc.contributor.authorChen, Xiaoruien_US
dc.contributor.authorNi, Fengyunen_US
dc.contributor.authorTian, Xiaen_US
dc.contributor.authorKondrashkina, Elenaen_US
dc.contributor.authorWang, Qinghuaen_US
dc.contributor.authorMa, Jianpengen_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2013-06-19T16:46:14Zen_US
dc.date.available2013-06-19T16:46:14Zen_US
dc.date.issued2013en_US
dc.description.abstractSpontaneous nucleation of actin is very inefficient in cells. To overcome this barrier, cells have evolved a set of actin filament nucleators to promote rapid nucleation and polymerization in response to specific stimuli. However, the molecular mechanism of actin nucleation remains poorly understood. This is hindered largely by the fact that actin nucleus, once formed, rapidly polymerizes into filament, thus making it impossible to capture stable multisubunit actin nucleus. Here, we report an effective doublemutant strategy to stabilize actin nucleus by preventing further polymerization. Employing this strategy, we solved the crystal structure of AMPPNP-actin in complex with the first two tandem W domains of Cordon-bleu (Cobl), a potent actin filament nucleator. Further sequence comparison and functional studies suggest that the nucleation mechanism of Cobl is probably shared by the p53 cofactor JMY, but not Spire. Moreover, the double-mutant strategy opens the way for atomic mechanistic study of actin nucleation and polymerization.en_US
dc.embargo.termsnoneen_US
dc.identifier.citationChen, Xiaorui, Ni, Fengyun, Tian, Xia, et al.. "Structural Basis of Actin Filament Nucleation by Tandem W Domains." <i>Cell Reports,</i> 3, (2013) Cell Press: 1-11. http://dx.doi.org/10.1016/j.celrep.2013.04.028.en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.celrep.2013.04.028en_US
dc.identifier.urihttps://hdl.handle.net/1911/71326en_US
dc.language.isoengen_US
dc.publisherCell Pressen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.titleStructural Basis of Actin Filament Nucleation by Tandem W Domainsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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