An open-hardware platform for optogenetics and photobiology

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dc.citation.articleNumber35363
dc.citation.journalTitleScientific Reports
dc.citation.volumeNumber6
dc.contributor.authorGerhardt, Karl P.
dc.contributor.authorOlson, Evan J.
dc.contributor.authorCastillo-Hair, Sebastian M.
dc.contributor.authorHartsough, Lucas A.
dc.contributor.authorLandry, Brian P.
dc.contributor.authorEkness, Felix
dc.contributor.authorYokoo, Rayka
dc.contributor.authorGomez, Eric J.
dc.contributor.authorRamakrishnan, Prabha
dc.contributor.authorSuh, Junghae
dc.contributor.authorSavage, David F.
dc.contributor.authorTabor, Jeffrey J.
dc.date.accessioned2017-05-19T20:48:13Z
dc.date.available2017-05-19T20:48:13Z
dc.date.issued2016
dc.description.abstractIn optogenetics, researchers use light and genetically encoded photoreceptors to control biological processes with unmatched precision. However, outside of neuroscience, the impact of optogenetics has been limited by a lack of user-friendly, flexible, accessible hardware. Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution. Signals are programmed using an intuitive web tool named Iris. All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day. We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells and simplify the entrainment of cyanobacterial circadian rhythm. The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
dc.identifier.citationGerhardt, Karl P., Olson, Evan J., Castillo-Hair, Sebastian M., et al.. "An open-hardware platform for optogenetics and photobiology." <i>Scientific Reports,</i> 6, (2016) Springer Nature: https://doi.org/10.1038/srep35363.
dc.identifier.doihttps://doi.org/10.1038/srep35363
dc.identifier.urihttps://hdl.handle.net/1911/94309
dc.language.isoeng
dc.publisherSpringer Nature
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the articleﾒs Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleAn open-hardware platform for optogenetics and photobiology
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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