An In Vivo Platform for Rebuilding Functional Neocortical Tissue

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dc.citation.articleNumber263en_US
dc.citation.issueNumber2en_US
dc.citation.journalTitleBioengineeringen_US
dc.citation.volumeNumber10en_US
dc.contributor.authorQuezada, Alexandraen_US
dc.contributor.authorWard, Claireen_US
dc.contributor.authorBader, Edward R.en_US
dc.contributor.authorZolotavin, Pavloen_US
dc.contributor.authorAltun, Esraen_US
dc.contributor.authorHong, Sarahen_US
dc.contributor.authorKillian, Nathaniel J.en_US
dc.contributor.authorXie, Chongen_US
dc.contributor.authorBatista-Brito, Renataen_US
dc.contributor.authorHébert, Jean M.en_US
dc.date.accessioned2023-03-10T19:04:12Zen_US
dc.date.available2023-03-10T19:04:12Zen_US
dc.date.issued2023en_US
dc.description.abstractRecent progress in cortical stem cell transplantation has demonstrated its potential to repair the brain. However, current transplant models have yet to demonstrate that the circuitry of transplant-derived neurons can encode useful function to the host. This is likely due to missing cell types within the grafts, abnormal proportions of cell types, abnormal cytoarchitecture, and inefficient vascularization. Here, we devised a transplant platform for testing neocortical tissue prototypes. Dissociated mouse embryonic telencephalic cells in a liquid scaffold were transplanted into aspiration-lesioned adult mouse cortices. The donor neuronal precursors differentiated into upper and deep layer neurons that exhibited synaptic puncta, projected outside of the graft to appropriate brain areas, became electrophysiologically active within one month post-transplant, and responded to visual stimuli. Interneurons and oligodendrocytes were present at normal densities in grafts. Grafts became fully vascularized by one week post-transplant and vessels in grafts were perfused with blood. With this paradigm, we could also organize cells into layers. Overall, we have provided proof of a concept for an in vivo platform that can be used for developing and testing neocortical-like tissue prototypes.en_US
dc.identifier.citationQuezada, Alexandra, Ward, Claire, Bader, Edward R., et al.. "An In Vivo Platform for Rebuilding Functional Neocortical Tissue." <i>Bioengineering,</i> 10, no. 2 (2023) MDPI: https://doi.org/10.3390/bioengineering10020263.en_US
dc.identifier.digitalbioengineering-10-00263-v2en_US
dc.identifier.doihttps://doi.org/10.3390/bioengineering10020263en_US
dc.identifier.urihttps://hdl.handle.net/1911/114500en_US
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleAn In Vivo Platform for Rebuilding Functional Neocortical Tissueen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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