An In Vivo Platform for Rebuilding Functional Neocortical Tissue

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dc.citation.articleNumber263
dc.citation.issueNumber2
dc.citation.journalTitleBioengineering
dc.citation.volumeNumber10
dc.contributor.authorQuezada, Alexandra
dc.contributor.authorWard, Claire
dc.contributor.authorBader, Edward R.
dc.contributor.authorZolotavin, Pavlo
dc.contributor.authorAltun, Esra
dc.contributor.authorHong, Sarah
dc.contributor.authorKillian, Nathaniel J.
dc.contributor.authorXie, Chong
dc.contributor.authorBatista-Brito, Renata
dc.contributor.authorHébert, Jean M.
dc.date.accessioned2023-03-10T19:04:12Z
dc.date.available2023-03-10T19:04:12Z
dc.date.issued2023
dc.description.abstractRecent progress in cortical stem cell transplantation has demonstrated its potential to repair the brain. However, current transplant models have yet to demonstrate that the circuitry of transplant-derived neurons can encode useful function to the host. This is likely due to missing cell types within the grafts, abnormal proportions of cell types, abnormal cytoarchitecture, and inefficient vascularization. Here, we devised a transplant platform for testing neocortical tissue prototypes. Dissociated mouse embryonic telencephalic cells in a liquid scaffold were transplanted into aspiration-lesioned adult mouse cortices. The donor neuronal precursors differentiated into upper and deep layer neurons that exhibited synaptic puncta, projected outside of the graft to appropriate brain areas, became electrophysiologically active within one month post-transplant, and responded to visual stimuli. Interneurons and oligodendrocytes were present at normal densities in grafts. Grafts became fully vascularized by one week post-transplant and vessels in grafts were perfused with blood. With this paradigm, we could also organize cells into layers. Overall, we have provided proof of a concept for an in vivo platform that can be used for developing and testing neocortical-like tissue prototypes.
dc.identifier.citationQuezada, Alexandra, Ward, Claire, Bader, Edward R., et al.. "An In Vivo Platform for Rebuilding Functional Neocortical Tissue." <i>Bioengineering,</i> 10, no. 2 (2023) MDPI: https://doi.org/10.3390/bioengineering10020263.
dc.identifier.digitalbioengineering-10-00263-v2
dc.identifier.doihttps://doi.org/10.3390/bioengineering10020263
dc.identifier.urihttps://hdl.handle.net/1911/114500
dc.language.isoeng
dc.publisherMDPI
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleAn In Vivo Platform for Rebuilding Functional Neocortical Tissue
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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