Mechanistic Understanding of the Phosphorylation-Induced Conformational Rigidity at the AMPA Receptor C-terminal Domain

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dc.citation.firstpage14211
dc.citation.issueNumber10
dc.citation.journalTitleACS Omega
dc.citation.lastpage14218
dc.citation.volumeNumber4
dc.contributor.authorChatterjee, Sudeshna
dc.contributor.authorDutta, Chayan
dc.contributor.authorCarrejo, Nicole C.
dc.contributor.authorLandes, Christy F.
dc.date.accessioned2019-10-25T16:54:17Z
dc.date.available2019-10-25T16:54:17Z
dc.date.issued2019
dc.description.abstractPhosphorylation at the intracellular C-terminal domain (CTD) of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors induces conformational rigidity. Such intracellular alterations to the AMPA receptor influence its functional responses, which are involved in multiple synaptic processes and neuronal signaling. The structure of the CTD still remains unresolved, which poses challenges toward providing a mechanism for the process of phosphorylation and deciphering the role of each phosphorylation step in causing the resultant conformational behavior. Herein, we utilize smFRET spectroscopy to understand the mechanism of phosphorylation, with the help of strategic point mutations that mimic phosphorylation. Our results reveal that first, phosphorylation at three target sites (S818, S831, and T840) is necessary for the change in the secondary structure of the existing disordered native sequence. Also, the results suggest that the formation of the tertiary structure through electrostatic interaction involving one specific phosphorylation site (S831) stabilizes the structure and renders conformational rigidity.
dc.identifier.citationChatterjee, Sudeshna, Dutta, Chayan, Carrejo, Nicole C., et al.. "Mechanistic Understanding of the Phosphorylation-Induced Conformational Rigidity at the AMPA Receptor C-terminal Domain." <i>ACS Omega,</i> 4, no. 10 (2019) American Chemical Society: 14211-14218. https://doi.org/10.1021/acsomega.9b01384.
dc.identifier.doihttps://doi.org/10.1021/acsomega.9b01384
dc.identifier.urihttps://hdl.handle.net/1911/107504
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.rightsThis article is distributed under the terms of the ACS AuthorChoice license
dc.rights.urihttps://pubs.acs.org/page/policy/authorchoice_termsofuse.html
dc.titleMechanistic Understanding of the Phosphorylation-Induced Conformational Rigidity at the AMPA Receptor C-terminal Domain
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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