Mechanistic Understanding of the Phosphorylation-Induced Conformational Rigidity at the AMPA Receptor C-terminal Domain

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dc.citation.firstpage14211en_US
dc.citation.issueNumber10en_US
dc.citation.journalTitleACS Omegaen_US
dc.citation.lastpage14218en_US
dc.citation.volumeNumber4en_US
dc.contributor.authorChatterjee, Sudeshnaen_US
dc.contributor.authorDutta, Chayanen_US
dc.contributor.authorCarrejo, Nicole C.en_US
dc.contributor.authorLandes, Christy F.en_US
dc.date.accessioned2019-10-25T16:54:17Zen_US
dc.date.available2019-10-25T16:54:17Zen_US
dc.date.issued2019en_US
dc.description.abstractPhosphorylation at the intracellular C-terminal domain (CTD) of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors induces conformational rigidity. Such intracellular alterations to the AMPA receptor influence its functional responses, which are involved in multiple synaptic processes and neuronal signaling. The structure of the CTD still remains unresolved, which poses challenges toward providing a mechanism for the process of phosphorylation and deciphering the role of each phosphorylation step in causing the resultant conformational behavior. Herein, we utilize smFRET spectroscopy to understand the mechanism of phosphorylation, with the help of strategic point mutations that mimic phosphorylation. Our results reveal that first, phosphorylation at three target sites (S818, S831, and T840) is necessary for the change in the secondary structure of the existing disordered native sequence. Also, the results suggest that the formation of the tertiary structure through electrostatic interaction involving one specific phosphorylation site (S831) stabilizes the structure and renders conformational rigidity.en_US
dc.identifier.citationChatterjee, Sudeshna, Dutta, Chayan, Carrejo, Nicole C., et al.. "Mechanistic Understanding of the Phosphorylation-Induced Conformational Rigidity at the AMPA Receptor C-terminal Domain." <i>ACS Omega,</i> 4, no. 10 (2019) American Chemical Society: 14211-14218. https://doi.org/10.1021/acsomega.9b01384.en_US
dc.identifier.doihttps://doi.org/10.1021/acsomega.9b01384en_US
dc.identifier.urihttps://hdl.handle.net/1911/107504en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis article is distributed under the terms of the ACS AuthorChoice licenseen_US
dc.rights.urihttps://pubs.acs.org/page/policy/authorchoice_termsofuse.htmlen_US
dc.titleMechanistic Understanding of the Phosphorylation-Induced Conformational Rigidity at the AMPA Receptor C-terminal Domainen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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