Rapid Stereomicroscopic Imaging of HER2 Overexpression in Ex Vivo Breast Tissue Using Topically Applied Silica-Based Gold Nanoshells

Simple item page
dc.citation.journalTitleJournal of Oncology
dc.citation.volumeNumber2012
dc.contributor.authorBickford, Lissett R.
dc.contributor.authorLangsner, Robert J.
dc.contributor.authorChang, Joseph
dc.contributor.authorKennedy, Laura C.
dc.contributor.authorAgollah, Germaine D.
dc.contributor.authorDrezek, Rebekah
dc.date.accessioned2013-03-20T18:11:34Z
dc.date.available2013-03-20T18:11:34Z
dc.date.issued2012
dc.description.abstractTumor margin detection for patients undergoing breast conservation surgery primarily occurs postoperatively. Previously, we demonstrated that gold nanoshells rapidly enhance contrast of HER2 overexpression in ex vivo tissue sections. Our ultimate objective, however, is to discern HER2 overexpressing tissue from normal tissue in whole, nonsectioned, specimens to facilitate rapid diagnoses. Here, we use targeted nanoshells to quickly and effectively visualize HER2 receptor expression in intact ex vivo human breast tissue specimens. Punch biopsies of human breast tissue were analyzed after a brief 5-minute incubation with and without HER2-targeted silica-gold nanoshells using two-photon microscopy and stereomicroscopy. Labeling was subsequently verified using reflectance confocal microscopy, darkfield hyperspectral imaging, and immunohistochemistry to confirm levels of HER2 expression. Our results suggest that anti-HER2 nanoshells used in tandem with a near-infrared reflectance confocal microscope and a standard stereomicroscope may potentially be used to discern HER2-overexpressing cancerous tissue from normal tissue in near real time and offer a rapid supplement to current diagnostic techniques.
dc.embargo.termsnone
dc.identifier.citationBickford, Lissett R., Langsner, Robert J., Chang, Joseph, et al.. "Rapid Stereomicroscopic Imaging of HER2 Overexpression in Ex Vivo Breast Tissue Using Topically Applied Silica-Based Gold Nanoshells." <i>Journal of Oncology,</i> 2012, (2012) Hindawi: http://dx.doi.org/10.1155/2012/291898.
dc.identifier.doihttp://dx.doi.org/10.1155/2012/291898
dc.identifier.otherArticle ID 291898
dc.identifier.urihttps://hdl.handle.net/1911/70790
dc.language.isoeng
dc.publisherHindawi
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.titleRapid Stereomicroscopic Imaging of HER2 Overexpression in Ex Vivo Breast Tissue Using Topically Applied Silica-Based Gold Nanoshells
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
stereomicroscopic-imaging.pdf
Size:
11.98 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Bioengineering Publications
ECE Publications
Faculty Publications