Quantification of Genome Editing and Transcriptional Control Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in Human Cells

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dc.citation.firstpage3239en_US
dc.citation.issueNumber10en_US
dc.citation.journalTitleACS Synthetic Biologyen_US
dc.citation.lastpage3250en_US
dc.citation.volumeNumber11en_US
dc.contributor.authorEscobar, Marioen_US
dc.contributor.authorLi, Jingen_US
dc.contributor.authorPatel, Aditien_US
dc.contributor.authorLiu, Shizheen_US
dc.contributor.authorXu, Qien_US
dc.contributor.authorHilton, Isaac B.en_US
dc.date.accessioned2022-12-13T19:11:16Zen_US
dc.date.available2022-12-13T19:11:16Zen_US
dc.date.issued2022en_US
dc.description.abstractCRISPR/Cas technologies have revolutionized the ability to redesign genomic information and tailor endogenous gene expression. Nevertheless, the discovery and development of new CRISPR/Cas systems has resulted in a lack of clarity surrounding the relative efficacies among these technologies in human cells. This deficit makes the optimal selection of CRISPR/Cas technologies in human cells unnecessarily challenging, which in turn hampers their adoption, and thus ultimately limits their utility. Here, we designed a series of endogenous testbed systems to methodically quantify and compare the genome editing, CRISPRi, and CRISPRa capabilities among 10 different natural and engineered Cas protein variants spanning Type II and Type V CRISPR/Cas families. We show that although all Cas protein variants are capable of genome editing and transcriptional control in human cells, hierarchies exist, particularly for genome editing and CRISPRa applications, wherein Cas9 ≥ Cas12a > Cas12e/Cas12j. Our findings also highlight the utility of our modular testbed platforms to rapidly and systematically quantify the functionality of practically any natural or engineered genomic-targeting Cas protein in human cells.en_US
dc.identifier.citationEscobar, Mario, Li, Jing, Patel, Aditi, et al.. "Quantification of Genome Editing and Transcriptional Control Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in Human Cells." <i>ACS Synthetic Biology,</i> 11, no. 10 (2022) American Chemical Society: 3239-3250. https://doi.org/10.1021/acssynbio.2c00156.en_US
dc.identifier.digitalacssynbio-2c00156en_US
dc.identifier.doihttps://doi.org/10.1021/acssynbio.2c00156en_US
dc.identifier.urihttps://hdl.handle.net/1911/114093en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis article is published under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.titleQuantification of Genome Editing and Transcriptional Control Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in Human Cellsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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