Development of photoreactive demineralized bone matrix 3D printing colloidal inks for bone tissue engineering

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dc.citation.articleNumberrbad090en_US
dc.citation.journalTitleRegenerative Biomaterialsen_US
dc.citation.volumeNumber10en_US
dc.contributor.authorHogan, Katie Jen_US
dc.contributor.authorÖztatlı, Hayriyeen_US
dc.contributor.authorPerez, Marissa Ren_US
dc.contributor.authorSi, Sophiaen_US
dc.contributor.authorUmurhan, Reyhanen_US
dc.contributor.authorJui, Elysaen_US
dc.contributor.authorWang, Ziwenen_US
dc.contributor.authorJiang, Emily Yen_US
dc.contributor.authorHan, Sa Ren_US
dc.contributor.authorDiba, Manien_US
dc.contributor.authorJane Grande-Allen, Ken_US
dc.contributor.authorGaripcan, Boraen_US
dc.contributor.authorMikos, Antonios Gen_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2024-05-03T15:51:14Zen_US
dc.date.available2024-05-03T15:51:14Zen_US
dc.date.issued2023en_US
dc.description.abstractDemineralized bone matrix (DBM) has been widely used clinically for dental, craniofacial and skeletal bone repair, as an osteoinductive and osteoconductive material. 3D printing (3DP) enables the creation of bone tissue engineering scaffolds with complex geometries and porosity. Photoreactive methacryloylated gelatin nanoparticles (GNP-MAs) 3DP inks have been developed, which display gel-like behavior for high print fidelity and are capable of post-printing photocrosslinking for control of scaffold swelling and degradation. Here, novel DBM nanoparticles (DBM-NPs, ∼400 nm) were fabricated and characterized prior to incorporation in 3DP inks. The objectives of this study were to determine how these DBM-NPs would influence the printability of composite colloidal 3DP inks, assess the impact of ultraviolet (UV) crosslinking on 3DP scaffold swelling and degradation and evaluate the osteogenic potential of DBM-NP-containing composite colloidal scaffolds. The addition of methacryloylated DBM-NPs (DBM-NP-MAs) to composite colloidal inks (100:0, 95:5 and 75:25 GNP-MA:DBM-NP-MA) did not significantly impact the rheological properties associated with printability, such as viscosity and shear recovery or photocrosslinking. UV crosslinking with a UV dosage of 3 J/cm2 directly impacted the rate of 3DP scaffold swelling for all GNP-MA:DBM-NP-MA ratios with an ∼40% greater increase in scaffold area and pore area in uncrosslinked versus photocrosslinked scaffolds over 21 days in phosphate-buffered saline (PBS). Likewise, degradation (hydrolytic and enzymatic) over 21 days for all DBM-NP-MA content groups was significantly decreased, ∼45% less in PBS and collagenase-containing PBS, in UV-crosslinked versus uncrosslinked groups. The incorporation of DBM-NP-MAs into scaffolds decreased mass loss compared to GNP-MA-only scaffolds during collagenase degradation. An in vitro osteogenic study with bone marrow-derived mesenchymal stem cells demonstrated osteoconductive properties of 3DP scaffolds for the DBM-NP-MA contents examined. The creation of photoreactive DBM-NP-MAs and their application in 3DP provide a platform for the development of ECM-derived colloidal materials and tailored control of biochemical cue presentation with broad tissue engineering applications.en_US
dc.identifier.citationHogan, K. J., Öztatlı, H., Perez, M. R., Si, S., Umurhan, R., Jui, E., Wang, Z., Jiang, E. Y., Han, S. R., Diba, M., Jane Grande-Allen, K., Garipcan, B., & Mikos, A. G. (2023). Development of photoreactive demineralized bone matrix 3D printing colloidal inks for bone tissue engineering. Regenerative Biomaterials, 10, rbad090. https://doi.org/10.1093/rb/rbad090en_US
dc.identifier.digitalrbad090en_US
dc.identifier.doihttps://doi.org/10.1093/rb/rbad090en_US
dc.identifier.urihttps://hdl.handle.net/1911/115581en_US
dc.language.isoengen_US
dc.publisherOxford University Pressen_US
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license. Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleDevelopment of photoreactive demineralized bone matrix 3D printing colloidal inks for bone tissue engineeringen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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