Versatile phenotype-activated cell sorting

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dc.citation.articleNumbereabb7438en_US
dc.citation.issueNumber43en_US
dc.citation.journalTitleScience Advancesen_US
dc.citation.volumeNumber6en_US
dc.contributor.authorLee, Jihwanen_US
dc.contributor.authorLiu, Zhuoheen_US
dc.contributor.authorSuzuki, Peter H.en_US
dc.contributor.authorAhrens, John F.en_US
dc.contributor.authorLai, Shujuanen_US
dc.contributor.authorLu, Xiaoyuen_US
dc.contributor.authorGuan, Sihuien_US
dc.contributor.authorSt-Pierre, Françoisen_US
dc.date.accessioned2020-11-17T19:44:48Zen_US
dc.date.available2020-11-17T19:44:48Zen_US
dc.date.issued2020en_US
dc.description.abstractUnraveling the genetic and epigenetic determinants of phenotypes is critical for understanding and re-engineering biology and would benefit from improved methods to separate cells based on phenotypes. Here, we report SPOTlight, a versatile high-throughput technique to isolate individual yeast or human cells with unique spatiotemporal profiles from heterogeneous populations. SPOTlight relies on imaging visual phenotypes by microscopy, precise optical tagging of single target cells, and retrieval of tagged cells by fluorescence-activated cell sorting. To illustrate SPOTlight’s ability to screen cells based on temporal properties, we chose to develop a photostable yellow fluorescent protein for extended imaging experiments. We screened 3 million cells expressing mutagenesis libraries and identified a bright new variant, mGold, that is the most photostable yellow fluorescent protein reported to date. We anticipate that the versatility of SPOTlight will facilitate its deployment to decipher the rules of life, understand diseases, and engineer new molecules and cellsen_US
dc.identifier.citationLee, Jihwan, Liu, Zhuohe, Suzuki, Peter H., et al.. "Versatile phenotype-activated cell sorting." <i>Science Advances,</i> 6, no. 43 (2020) AAAS: https://doi.org/10.1126/sciadv.abb7438.en_US
dc.identifier.doihttps://doi.org/10.1126/sciadv.abb7438en_US
dc.identifier.urihttps://hdl.handle.net/1911/109568en_US
dc.language.isoengen_US
dc.publisherAAASen_US
dc.rightsDistributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/en_US
dc.titleVersatile phenotype-activated cell sortingen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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