Versatile phenotype-activated cell sorting

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dc.citation.articleNumbereabb7438
dc.citation.issueNumber43
dc.citation.journalTitleScience Advances
dc.citation.volumeNumber6
dc.contributor.authorLee, Jihwan
dc.contributor.authorLiu, Zhuohe
dc.contributor.authorSuzuki, Peter H.
dc.contributor.authorAhrens, John F.
dc.contributor.authorLai, Shujuan
dc.contributor.authorLu, Xiaoyu
dc.contributor.authorGuan, Sihui
dc.contributor.authorSt-Pierre, François
dc.date.accessioned2020-11-17T19:44:48Z
dc.date.available2020-11-17T19:44:48Z
dc.date.issued2020
dc.description.abstractUnraveling the genetic and epigenetic determinants of phenotypes is critical for understanding and re-engineering biology and would benefit from improved methods to separate cells based on phenotypes. Here, we report SPOTlight, a versatile high-throughput technique to isolate individual yeast or human cells with unique spatiotemporal profiles from heterogeneous populations. SPOTlight relies on imaging visual phenotypes by microscopy, precise optical tagging of single target cells, and retrieval of tagged cells by fluorescence-activated cell sorting. To illustrate SPOTlight’s ability to screen cells based on temporal properties, we chose to develop a photostable yellow fluorescent protein for extended imaging experiments. We screened 3 million cells expressing mutagenesis libraries and identified a bright new variant, mGold, that is the most photostable yellow fluorescent protein reported to date. We anticipate that the versatility of SPOTlight will facilitate its deployment to decipher the rules of life, understand diseases, and engineer new molecules and cells
dc.identifier.citationLee, Jihwan, Liu, Zhuohe, Suzuki, Peter H., et al.. "Versatile phenotype-activated cell sorting." <i>Science Advances,</i> 6, no. 43 (2020) AAAS: https://doi.org/10.1126/sciadv.abb7438.
dc.identifier.doihttps://doi.org/10.1126/sciadv.abb7438
dc.identifier.urihttps://hdl.handle.net/1911/109568
dc.language.isoeng
dc.publisherAAAS
dc.rightsDistributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.titleVersatile phenotype-activated cell sorting
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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