Stargazin Modulation of AMPA Receptors

dc.citation.firstpage328en_US
dc.citation.issueNumber2en_US
dc.citation.journalTitleCell Reportsen_US
dc.citation.lastpage335en_US
dc.citation.volumeNumber17en_US
dc.contributor.authorShaikh, Sana A.en_US
dc.contributor.authorDolino, Drew M.en_US
dc.contributor.authorLee, Garamen_US
dc.contributor.authorChatterjee, Sudeshnaen_US
dc.contributor.authorMacLean, David M.en_US
dc.contributor.authorFlatebo, Charlotteen_US
dc.contributor.authorLandes, Christy F.en_US
dc.contributor.authorJayaraman, Vasanthien_US
dc.contributor.orgApplied Physics Graduate Programen_US
dc.date.accessioned2016-12-01T17:29:09Z
dc.date.available2016-12-01T17:29:09Z
dc.date.issued2016en_US
dc.description.abstractFast excitatory synaptic signaling in the mammalian brain is mediated by AMPA-type ionotropic glutamate receptors. In neurons, AMPA receptors co-assemble with auxiliary proteins, such as stargazin, which can markedly alter receptor trafficking and gating. Here, we used luminescence resonance energy transfer measurements to map distances between the full-length, functional AMPA receptor and stargazin expressed in HEK293 cells and to determine the ensemble structural changes in the receptor due to stargazin. In addition, we used single-molecule fluorescence resonance energy transfer to study the structural and conformational distribution of the receptor and how this distribution is affected by stargazin. Our nanopositioning data place stargazin below the AMPA receptor ligand-binding domain, where it is well poised to act as a scaffold to facilitate the long-range conformational selection observations seen in single-molecule experiments. These data support a model of stargazin acting to stabilize or select conformational states that favor activation.en_US
dc.identifier.citationShaikh, Sana A., Dolino, Drew M., Lee, Garam, et al.. "Stargazin Modulation of AMPA Receptors." <i>Cell Reports,</i> 17, no. 2 (2016) Elsevier: 328-335. http://dx.doi.org/10.1016/j.celrep.2016.09.014.
dc.identifier.doihttp://dx.doi.org/10.1016/j.celrep.2016.09.014en_US
dc.identifier.urihttps://hdl.handle.net/1911/92735
dc.language.isoengen_US
dc.publisherElsevier
dc.rightsThis is an open access article under the CC BY-NC-ND licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subject.keywordAMPA receptorsen_US
dc.subject.keywordtransmembrane AMPA receptor regulatory proteinsen_US
dc.subject.keywordstargazingen_US
dc.subject.keywordAMPA receptor modulationen_US
dc.subject.keyworddesensitized stateen_US
dc.subject.keywordsmFRETen_US
dc.titleStargazin Modulation of AMPA Receptorsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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