Stargazin Modulation of AMPA Receptors

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dc.citation.firstpage328
dc.citation.issueNumber2
dc.citation.journalTitleCell Reports
dc.citation.lastpage335
dc.citation.volumeNumber17
dc.contributor.authorShaikh, Sana A.
dc.contributor.authorDolino, Drew M.
dc.contributor.authorLee, Garam
dc.contributor.authorChatterjee, Sudeshna
dc.contributor.authorMacLean, David M.
dc.contributor.authorFlatebo, Charlotte
dc.contributor.authorLandes, Christy F.
dc.contributor.authorJayaraman, Vasanthi
dc.contributor.orgApplied Physics Graduate Program
dc.date.accessioned2016-12-01T17:29:09Z
dc.date.available2016-12-01T17:29:09Z
dc.date.issued2016
dc.description.abstractFast excitatory synaptic signaling in the mammalian brain is mediated by AMPA-type ionotropic glutamate receptors. In neurons, AMPA receptors co-assemble with auxiliary proteins, such as stargazin, which can markedly alter receptor trafficking and gating. Here, we used luminescence resonance energy transfer measurements to map distances between the full-length, functional AMPA receptor and stargazin expressed in HEK293 cells and to determine the ensemble structural changes in the receptor due to stargazin. In addition, we used single-molecule fluorescence resonance energy transfer to study the structural and conformational distribution of the receptor and how this distribution is affected by stargazin. Our nanopositioning data place stargazin below the AMPA receptor ligand-binding domain, where it is well poised to act as a scaffold to facilitate the long-range conformational selection observations seen in single-molecule experiments. These data support a model of stargazin acting to stabilize or select conformational states that favor activation.
dc.identifier.citationShaikh, Sana A., Dolino, Drew M., Lee, Garam, et al.. "Stargazin Modulation of AMPA Receptors." <i>Cell Reports,</i> 17, no. 2 (2016) Elsevier: 328-335. http://dx.doi.org/10.1016/j.celrep.2016.09.014.
dc.identifier.doihttp://dx.doi.org/10.1016/j.celrep.2016.09.014
dc.identifier.urihttps://hdl.handle.net/1911/92735
dc.language.isoeng
dc.publisherElsevier
dc.rightsThis is an open access article under the CC BY-NC-ND license
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keywordAMPA receptors
dc.subject.keywordtransmembrane AMPA receptor regulatory proteins
dc.subject.keywordstargazing
dc.subject.keywordAMPA receptor modulation
dc.subject.keyworddesensitized state
dc.subject.keywordsmFRET
dc.titleStargazin Modulation of AMPA Receptors
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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