Mycoplasma contamination of leukemic cell lines alters protein expression determined by reverse phase protein arrays

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dc.citation.journalTitleCytotechnologyen_US
dc.contributor.authorHoff, Fieke W.en_US
dc.contributor.authorHu, Chenyue W.en_US
dc.contributor.authorQutub, Amina A.en_US
dc.contributor.authorQiu, Yihuaen_US
dc.contributor.authorGraver, Elizabethen_US
dc.contributor.authorHoang, Giangen_US
dc.contributor.authorChauhan, Manasien_US
dc.contributor.authorde Bont, Eveline S.J.M.en_US
dc.contributor.authorKornblau, Steven M.en_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2018-11-12T15:39:53Zen_US
dc.date.available2018-11-12T15:39:53Zen_US
dc.date.issued2018en_US
dc.description.abstractMycoplasma contamination is a major problem in cell culturing, potentially altering the results of cell line-based experiments in largely uncharacterized ways. To define the consequences of mycoplasma infection at the level of protein expression we utilized the reverse phase protein array technology to analyze the expression of 235 proteins in mycoplasma infected, uninfected post treatment, and never-infected leukemic cell lines. Overall, protein profiles of cultured cells remained relatively stable after mycoplasma infection. However, paired comparisons for individual proteins identified that 18.7% of the proteins significantly changed between the infected and the never-infected cell line samples, and that 14.0% of the proteins significantly altered between the infected and the post treatment samples. Six percent of the proteins were affected in the post treatment samples compared to the never-infected samples, and 7.2% compared to treated cells that had never had mycoplasma infection before. Proteins that were significantly altered in the infected cells were enriched for apoptotic signaling processes and auto-phosphorylation, suggesting an increased cellular stress and a decreased growth rate. In conclusion, this study shows that mycoplasma infection of leukemic cell lines alters the proteins expression levels, potentially confounding experimental results. This reinforces the need for regular testing of mycoplasma.en_US
dc.identifier.citationHoff, Fieke W., Hu, Chenyue W., Qutub, Amina A., et al.. "Mycoplasma contamination of leukemic cell lines alters protein expression determined by reverse phase protein arrays." <i>Cytotechnology,</i> (2018) Springer: https://doi.org/10.1007/s10616-018-0244-2.en_US
dc.identifier.digitalHoff2018en_US
dc.identifier.doihttps://doi.org/10.1007/s10616-018-0244-2en_US
dc.identifier.urihttps://hdl.handle.net/1911/103316en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordCell linesen_US
dc.subject.keywordMycoplasmaen_US
dc.subject.keywordProteomicsen_US
dc.subject.keywordRPPAen_US
dc.titleMycoplasma contamination of leukemic cell lines alters protein expression determined by reverse phase protein arraysen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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