Enhanced osteogenesis in co-cultures with human mesenchymal stem cells and endothelial cells on polymeric microfiber scaffolds

Simple item page
dc.citation.journalTitleTissue Engineering: Part A
dc.contributor.authorGershovich, Julia G.
dc.contributor.authorDahlin, Rebecca L.
dc.contributor.authorKasper, F. Kurtis
dc.contributor.authorMikos, Antonios G.
dc.date.accessioned2013-07-12T21:10:01Z
dc.date.available2013-07-12T21:10:01Z
dc.date.issued2013
dc.description.abstractIn this work, human mesenchymal stem cells (hMSCs) and their osteogenically precultured derivatives were directly co-cultured with human umbilical vein endothelial cells (HUVECs) on electrospun 3D poly(-caprolactone) microfiber scaffolds in order to evaluate the co-culture’s effect on the generation of osteogenic constructs. Specifically, cells were cultured on scaffolds for up to three weeks, and the cellularity, alkaline phosphatase activity (ALP), and bone-like matrix formation were assessed. Constructs with co-cultures and monocultures had almost identical cellularity after the first week, however lower cellularity was observed in co-cultures compared to monocultures during the subsequent two weeks of culture. Scaffolds with co-cultures showed significantly higher ALP activity, glycosaminoglycan and collagen production, as well as greater calcium deposition over the course of study compared to monocultures of hMSCs. Furthermore, the osteogenic outcome was equally robust in co-cultures containing osteogenically precultured and non-precultured hMSCs. The results demonstrate that the combination of MSC and HUVEC populations within a porous scaffold material under osteogenic culture conditions is an effective strategy to promote osteogenesis.
dc.embargo.termsnone
dc.identifier.citationGershovich, Julia G., Dahlin, Rebecca L., Kasper, F. Kurtis, et al.. "Enhanced osteogenesis in co-cultures with human mesenchymal stem cells and endothelial cells on polymeric microfiber scaffolds." <i>Tissue Engineering: Part A,</i> (2013) Mary Ann Liebert, Inc.: http://dx.doi.org/10.1089/ten.TEA.2013.0256.
dc.identifier.doihttp://dx.doi.org/10.1089/ten.TEA.2013.0256
dc.identifier.urihttps://hdl.handle.net/1911/71555
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc.
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Mary Ann Liebert, Inc.
dc.subject.keywordbone tissue engineering
dc.subject.keywordco-culture
dc.subject.keywordmesenchymal stem cells
dc.subject.keywordhuman umbilical vein endothelial cells
dc.subject.keyword3D culture
dc.subject.keywordscaffold
dc.titleEnhanced osteogenesis in co-cultures with human mesenchymal stem cells and endothelial cells on polymeric microfiber scaffolds
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Enhanced-osteogenesis.pdf
Size:
4.28 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Bioengineering Publications
Faculty Publications