Elevated Hoxb5b Expands Vagal Neural Crest Pool and Blocks Enteric Neuronal Development in Zebrafish

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dc.citation.articleNumber803370en_US
dc.citation.journalTitleFrontiers in Cell and Developmental Biologyen_US
dc.citation.volumeNumber9en_US
dc.contributor.authorHoward, Aubrey G. A.en_US
dc.contributor.authorNguyen, Aaron C.en_US
dc.contributor.authorTworig, Joshuaen_US
dc.contributor.authorRavisankar, Priyaen_US
dc.contributor.authorSingleton, Eileen W.en_US
dc.contributor.authorLi, Canen_US
dc.contributor.authorKotzur, Graysonen_US
dc.contributor.authorWaxman, Joshua S.en_US
dc.contributor.authorUribe, Rosa A.en_US
dc.date.accessioned2022-03-24T13:31:40Zen_US
dc.date.available2022-03-24T13:31:40Zen_US
dc.date.issued2022en_US
dc.description.abstractNeural crest cells (NCCs) are a migratory, transient, and multipotent stem cell population essential to vertebrate embryonic development, contributing to numerous cell lineages in the adult organism. While great strides have been made in elucidating molecular and cellular events that drive NCC specification, comprehensive knowledge of the genetic factors that orchestrate NCC developmental programs is still far from complete. We discovered that elevated Hoxb5b levels promoted an expansion of zebrafish NCCs, which persisted throughout multiple stages of development. Correspondingly, elevated Hoxb5b also specifically expanded expression domains of the vagal NCC markers foxd3 and phox2bb. Increases in NCCs were most apparent after pulsed ectopic Hoxb5b expression at early developmental stages, rather than later during differentiation stages, as determined using a novel transgenic zebrafish line. The increase in vagal NCCs early in development led to supernumerary Phox2b+ enteric neural progenitors, while leaving many other NCC-derived tissues without an overt phenotype. Surprisingly, these NCC-derived enteric progenitors failed to expand properly into sufficient quantities of enterically fated neurons and stalled in the gut tissue. These results suggest that while Hoxb5b participates in vagal NCC development as a driver of progenitor expansion, the supernumerary, ectopically localized NCC fail to initiate expansion programs in timely fashion in the gut. All together, these data point to a model in which Hoxb5b regulates NCCs both in a tissue specific and temporally restricted manner.en_US
dc.identifier.citationHoward, Aubrey G. A., Nguyen, Aaron C., Tworig, Joshua, et al.. "Elevated Hoxb5b Expands Vagal Neural Crest Pool and Blocks Enteric Neuronal Development in Zebrafish." <i>Frontiers in Cell and Developmental Biology,</i> 9, (2022) Frontiers Media S.A.: https://doi.org/10.3389/fcell.2021.803370.en_US
dc.identifier.digitalfcell-09-803370en_US
dc.identifier.doihttps://doi.org/10.3389/fcell.2021.803370en_US
dc.identifier.urihttps://hdl.handle.net/1911/112041en_US
dc.language.isoengen_US
dc.publisherFrontiers Media S.A.en_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleElevated Hoxb5b Expands Vagal Neural Crest Pool and Blocks Enteric Neuronal Development in Zebrafishen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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