Lactose repressor proteins with increased operator DNA binding affinity

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dc.contributor.assigneeRice Universityen_US
dc.contributor.publisherUnited States Patent and Trademark Officeen_US
dc.creatorMatthews, Kathleen S.en_US
dc.creatorFoster, Catherine M.en_US
dc.creatorSwint-kruse, Liskinen_US
dc.date.accessioned2015-05-04T19:05:47Zen_US
dc.date.available2015-05-04T19:05:47Zen_US
dc.date.filed2002-07-17en_US
dc.date.issued2008-06-24en_US
dc.description.abstractThe present invention provides altered lac repressor proteins that recognize the lactose operator with increased affinity and have either normal or enhanced ligand responsivity. For example, the lac repressor Gln60Gly mutant protein exhibits increased binding affinity for lactose operator DNA, while maintaining near-normal responsivity to IPTG. Alternatively, the present invention provides modified repressors which exhibit responsiveness to an alternative ligand, such as arabinose, or have enhanced responsivity to IPTG. For example, Gln60Gly/Leu148Phe binds with wild-type affinity to lactose operator DNA and exhibits enhanced responsivity to IPTG. The present invention also provides for repressors that exhibit both characteristics: increased affinity for lactose operator and enhanced ligand responsivity. Enhanced ligand response enables induction of gene expression to be finely controlled by a researcher. DNA sequences encoding the altered lac repressor proteins and bacterial and eukaryotic cells containing altered lac repressor proteins are also provided.en_US
dc.digitization.specificationsThis patent information was downloaded from the US Patent and Trademark website (http://www.uspto.gov/) as image-PDFs. The PDFs were OCRed for access purposes.en_US
dc.format.extent20 ppen_US
dc.identifier.citationMatthews, Kathleen S., Foster, Catherine M. and Swint-kruse, Liskin, "Lactose repressor proteins with increased operator DNA binding affinity." Patent US7390645B2. issued 2008-06-24. Retrieved from https://hdl.handle.net/1911/79971.en_US
dc.identifier.patentIDUS7390645B2en_US
dc.identifier.urihttps://hdl.handle.net/1911/79971en_US
dc.language.isoengen_US
dc.titleLactose repressor proteins with increased operator DNA binding affinityen_US
dc.typeUtility patenten_US
dc.type.dcmiTexten_US
dc.type.genrepatentsen_US
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