Quantitative Analysis of α-Synuclein Solubility in Living Cells Using Split GFP Complementation

Simple item page
dc.citation.firstpagee43505en_US
dc.citation.issueNumber8en_US
dc.citation.journalTitlePLoS ONEen_US
dc.citation.volumeNumber7en_US
dc.contributor.authorKothawala, Ahmeden_US
dc.contributor.authorKilpatrick, Kirien_US
dc.contributor.authorNovoa, Jose Andresen_US
dc.contributor.authorSegatori, Lauraen_US
dc.contributor.orgBioengineeringen_US
dc.contributor.orgBiosciencesen_US
dc.contributor.orgChemical and Biomolecular Engineeringen_US
dc.date.accessioned2015-01-06T19:20:34Zen_US
dc.date.available2015-01-06T19:20:34Zen_US
dc.date.issued2012en_US
dc.description.abstractPresently incurable, Parkinson's disease (PD) is the most common neurodegenerative movement disorder and affects 1% of the population over 60 years of age. The hallmarks of PD pathogenesis are the loss of dopaminergic neurons in the substantia nigra pars compacta, and the occurrence of proteinaceous cytoplasmic inclusions (Lewy bodies) in surviving neurons. Lewy bodies are mainly composed of the pre-synaptic protein alpha-synuclein (αsyn), an intrinsically unstructured, misfolding-prone protein with high propensity to aggregate. Quantifying the pool of soluble αsyn and monitoring αsyn aggregation in living cells is fundamental to study the molecular mechanisms of αsyn-induced cytotoxicity and develop therapeutic strategies to prevent αsyn aggregation. In this study, we report the use of a split GFP complementation assay to quantify αsyn solubility. Particularly, we investigated a series of naturally occurring and rationally designed αsyn variants and showed that this method can be used to study how αsyn sequence specificity affects its solubility. Furthermore, we demonstrated the utility of this assay to explore the influence of the cellular folding network on αsyn solubility. The results presented underscore the utility of the split GFP assay to quantify αsyn solubility in living cells.en_US
dc.identifier.citationKothawala, Ahmed, Kilpatrick, Kiri, Novoa, Jose Andres, et al.. "Quantitative Analysis of α-Synuclein Solubility in Living Cells Using Split GFP Complementation." <i>PLoS ONE,</i> 7, no. 8 (2012) Public Library of Science: e43505. http://dx.doi.org/10.1371/journal.pone.0043505.en_US
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0043505en_US
dc.identifier.urihttps://hdl.handle.net/1911/78897en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.titleQuantitative Analysis of α-Synuclein Solubility in Living Cells Using Split GFP Complementationen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
journal.pone.0043505.pdf
Size:
2.25 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications