Real-time hyperspectral fluorescence imaging of pancreatic b-cell dynamics with the image mapping spectrometer

dc.citation.firstpage4833en_US
dc.citation.journalTitleJournal of Cell Scienceen_US
dc.citation.lastpage4840en_US
dc.citation.volumeNumber125en_US
dc.contributor.authorElliott, Amicia D.en_US
dc.contributor.authorGao, Liangen_US
dc.contributor.authorUstione, Alessandroen_US
dc.contributor.authorBedard, Noahen_US
dc.contributor.authorKester, Roberten_US
dc.contributor.authorPiston, David W.en_US
dc.contributor.authorTkaczyk, Tomasz S.en_US
dc.date.accessioned2013-08-22T22:00:01Zen_US
dc.date.available2013-08-22T22:00:01Zen_US
dc.date.issued2012en_US
dc.description.abstractThe development of multi-colored fluorescent proteins, nanocrystals and organic fluorophores, along with the resulting engineered biosensors, has revolutionized the study of protein localization and dynamics in living cells. Hyperspectral imaging has proven to be a useful approach for such studies, but this technique is often limited by low signal and insufficient temporal resolution. Here, we present an implementation of a snapshot hyperspectral imaging device, the image mapping spectrometer (IMS), which acquires full spectral information simultaneously from each pixel in the field without scanning. The IMS is capable of real-time signal capture from multiple fluorophores with high collection efficiency (,65%) and image acquisition rate (up to 7.2 fps). To demonstrate the capabilities of the IMS in cellular applications, we have combined fluorescent protein (FP)-FRET and [Ca2+]i biosensors to measure simultaneously intracellular cAMP and [Ca2+]i signaling in pancreatic b-cells. Additionally, we have compared quantitatively the IMS detection efficiency with a laser-scanning confocal microscope.en_US
dc.embargo.termsnoneen_US
dc.identifier.citationElliott, Amicia D., Gao, Liang, Ustione, Alessandro, et al.. "Real-time hyperspectral fluorescence imaging of pancreatic b-cell dynamics with the image mapping spectrometer." <i>Journal of Cell Science,</i> 125, (2012) The Company of Biologists Ltd: 4833-4840. http://dx.doi.org/10.1242/jcs.108258.en_US
dc.identifier.doihttp://dx.doi.org/10.1242/jcs.108258en_US
dc.identifier.urihttps://hdl.handle.net/1911/71758en_US
dc.language.isoengen_US
dc.publisherThe Company of Biologists Ltden_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.subject.keywordhyperspectral imagingen_US
dc.subject.keywordfluorescent biosensorsen_US
dc.subject.keywordimage mapping spectrometeren_US
dc.subject.keywordIMSen_US
dc.titleReal-time hyperspectral fluorescence imaging of pancreatic b-cell dynamics with the image mapping spectrometeren_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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