Fine-tuned ultraspecific nucleic acid hybridization probes

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dc.contributor.assigneeRice University
dc.contributor.publisherUnited States Patent and Trademark Office
dc.creatorZhang, David Yu
dc.creatorWang, Juexiao
dc.creatorWu, Ruojia
dc.date.accessioned2021-03-02T23:50:08Z
dc.date.available2021-03-02T23:50:08Z
dc.date.filed2016-06-06
dc.date.issued2021-01-26
dc.description.abstractCompositions and methods for highly specific nucleic acid probes and primers are provided. The probe system comprises a complement strand and a protector stand that form a partially double-stranded probe. The reaction standard free energy of hybridization between the probe and target nucleic acid as determined by Expression 1 (ΔG°rxn=ΔG°t-TC−ΔG°nh-PC+(ΔG°v-TC−ΔG°h-PC)) is from about −4 kcal/mol to about +4 kcal/mol. Alternatively, the reaction standard free energy of hybridization between the probe and target nucleic acid is determined by Expression 1 to be within 5 kcal/mol of the standard free energy as determined by Expression 2 (−Rτ ln(([P]0−[C]0)/[C]0)]), where the [P]0 term of Expression 2 equals the concentration of the protector strand and the [C]0 term of Expression 2 equals the concentration of the complement strand. In addition, a method for on-the-fly fine tuning of a reaction using the present probe is provided.
dc.digitization.specificationsThis patent information was downloaded from the US Patent and Trademark website (http://www.uspto.gov/) as image-PDFs. The PDFs were OCRed for access purposes
dc.format.extent56
dc.identifier.citationZhang, David Yu, Wang, Juexiao and Wu, Ruojia, "Fine-tuned ultraspecific nucleic acid hybridization probes." Patent US10900079B2. issued 2021-01-26. Retrieved from <a href="https://hdl.handle.net/1911/110130">https://hdl.handle.net/1911/110130</a>.
dc.identifier.patentIDUS10900079B2
dc.identifier.urihttps://hdl.handle.net/1911/110130
dc.language.isoeng
dc.titleFine-tuned ultraspecific nucleic acid hybridization probes
dc.typeUtility patent
dc.type.dcmiText
dc.type.genrepatents
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