Label-free as-grown double wall carbon nanotubes bundles for Salmonella typhimurium immunoassay

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dc.citation.firstpage102en_US
dc.citation.journalTitleChemistry Central Journalen_US
dc.citation.volumeNumber7en_US
dc.contributor.authorPunbusayakul, Niramolen_US
dc.contributor.authorTalapatra, Saikaten_US
dc.contributor.authorAjayan, Pulickel Men_US
dc.contributor.authorSurareungchai, Werasaken_US
dc.date.accessioned2013-06-19T17:08:05Zen_US
dc.date.available2013-06-19T17:08:05Zen_US
dc.date.issued2013en_US
dc.description.abstractBackground: A label-free immunosensor from as-grown double wall carbon nanotubes (DW) bundles was developed for detecting Salmonella typhimurium. The immunosensor was fabricated by using the as-grown DW bundles as an electrode material with an anti-Salmonella impregnated on the surface. The immunosensor was electrochemically characterized by cyclic voltammetry. The working potential (100, 200, 300 and 400 mV vs. Ag/AgCl) and the anti-Salmonella concentration (10, 25, 50, 75, and 100 μg/mL) at the electrode were subsequently optimized. Then, chronoamperometry was used with the optimum potential of 100 mV vs. Ag/AgCl) and the optimum impregnated anti-Salmonella of 10 μg/mL to detect S. typhimurium cells (0-109 CFU/mL). Results: The DW immunosensor exhibited a detection range of 102 to 107 CFU/mL for the bacteria with a limit of detection of 8.9 CFU/mL according to the IUPAC recommendation. The electrode also showed specificity to S. typhimurium but no current response to Escherichia coli. Conclusions: These findings suggest that the use of a label-free DW immunosensor is promising for detecting S. typhimurium.en_US
dc.embargo.termsnoneen_US
dc.identifier.citationPunbusayakul, Niramol, Talapatra, Saikat, Ajayan, Pulickel M, et al.. "Label-free as-grown double wall carbon nanotubes bundles for Salmonella typhimurium immunoassay." <i>Chemistry Central Journal,</i> 7, (2013) Chemistry Central: 102. http://dx.doi.org/10.1186/1752-153X-7-102.en_US
dc.identifier.doihttp://dx.doi.org/10.1186/1752-153X-7-102en_US
dc.identifier.urihttps://hdl.handle.net/1911/71330en_US
dc.language.isoengen_US
dc.publisherChemistry Centralen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.titleLabel-free as-grown double wall carbon nanotubes bundles for Salmonella typhimurium immunoassayen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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