Label-free as-grown double wall carbon nanotubes bundles for Salmonella typhimurium immunoassay
| dc.citation.firstpage | 102 | |
| dc.citation.journalTitle | Chemistry Central Journal | |
| dc.citation.volumeNumber | 7 | |
| dc.contributor.author | Punbusayakul, Niramol | |
| dc.contributor.author | Talapatra, Saikat | |
| dc.contributor.author | Ajayan, Pulickel M | |
| dc.contributor.author | Surareungchai, Werasak | |
| dc.date.accessioned | 2013-06-19T17:08:05Z | |
| dc.date.available | 2013-06-19T17:08:05Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | Background: A label-free immunosensor from as-grown double wall carbon nanotubes (DW) bundles was developed for detecting Salmonella typhimurium. The immunosensor was fabricated by using the as-grown DW bundles as an electrode material with an anti-Salmonella impregnated on the surface. The immunosensor was electrochemically characterized by cyclic voltammetry. The working potential (100, 200, 300 and 400 mV vs. Ag/AgCl) and the anti-Salmonella concentration (10, 25, 50, 75, and 100 μg/mL) at the electrode were subsequently optimized. Then, chronoamperometry was used with the optimum potential of 100 mV vs. Ag/AgCl) and the optimum impregnated anti-Salmonella of 10 μg/mL to detect S. typhimurium cells (0-109 CFU/mL). Results: The DW immunosensor exhibited a detection range of 102 to 107 CFU/mL for the bacteria with a limit of detection of 8.9 CFU/mL according to the IUPAC recommendation. The electrode also showed specificity to S. typhimurium but no current response to Escherichia coli. Conclusions: These findings suggest that the use of a label-free DW immunosensor is promising for detecting S. typhimurium. | |
| dc.embargo.terms | none | |
| dc.identifier.citation | Punbusayakul, Niramol, Talapatra, Saikat, Ajayan, Pulickel M, et al.. "Label-free as-grown double wall carbon nanotubes bundles for Salmonella typhimurium immunoassay." <i>Chemistry Central Journal,</i> 7, (2013) Chemistry Central: 102. http://dx.doi.org/10.1186/1752-153X-7-102. | |
| dc.identifier.doi | http://dx.doi.org/10.1186/1752-153X-7-102 | |
| dc.identifier.uri | https://hdl.handle.net/1911/71330 | |
| dc.language.iso | eng | |
| dc.publisher | Chemistry Central | |
| dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | |
| dc.title | Label-free as-grown double wall carbon nanotubes bundles for Salmonella typhimurium immunoassay | |
| dc.type | Journal article | |
| dc.type.dcmi | Text | |
| dc.type.publication | publisher version |